10 May 2023 1 6K Report

the company that I'm working in produces sauce and we notice there is watery issue in our oyster sauce after bottling although the sauce is within its validated shelf life.

More Tharshini S Mahendran's questions See All
What is considered a biological replicate when you are doing an ELISA plate measurement with different cell types?

If cells obtained from different passages, with different plating but harvested on the same day are used in a single plate to take an ELISA measurement (with the given standards and treated...

17 June 2024 6,705 1 View

Which phosphor antibodies to choose to study the hypo and hyperphosphorylation state of the SRSF1 protein?

I am trying to understand the different phosphorylation states of SRSF1 protein in the cytoplasm vs nucleus and looking for specific antibodies for the phosphor state. As SR proteins include...

11 February 2024 7,242 3 View

Is it necessary to change media after transfecting cells with Lipofectamine3000?

I transfected HCt116 cells with GFP using Lipo3000. I didn't change media after transfection as the manufacturer guidelines say not necessary. However, I noticed a change in the cell morphology...

28 April 2023 6,071 6 View

Tafel analysis clarification and its reference?

I have done potentiodynamic polarization (Tafel) studies for a few surface modified samples. The intercepts and slopes were done exactly by the origin software. Its results show a gradual...

06 April 2023 1,226 1 View

Is Native RNA immunoprecipitation performed without cross linking?

I am trying to study the RNA-protein interactome of a specific protein using IP studies. I need to use native IP for my purpose and wanted to know if native RIP is without cross-linking study and...

01 February 2023 915 0 View

Is loading equal volume of protein in SDS PAGE correct?

I am comparing the protein expression levels in subcellular fractionations (Cytoplasm and nuclear). If I load an equal volume of both extracts (15 ul each to individual wells), is this still valid...

26 January 2023 6,257 3 View

How to use CRISPOR software for design guide RNA?

I want to know tutorial help to design and how to choose suitable g for knok out

29 July 2022 4,099 2 View

Which region of the gene should I target for a CRISPR mediated Knockout of Target gene?

I want to design g RNA for knock out pathway gene to enhance secondary metabolites

29 July 2022 8,077 0 View

What is the right media for HCT116?

Is DMEM or DMEM/F-12K the right media for culturing HCT116 cells apart from McCoys media? Did anyone use the gibco one? I see a lot of dead cells when using DMEM. It would be helpful if someone...

20 June 2022 1,059 3 View

How is FPKM calculated from raw gene count file?

I am working with RNAseq and qRT-PCR validation results to see a correlation. I am comparing a diseased group to a healthy group of cells. I have 4 biological replicates in each category. However,...

03 June 2022 7,982 5 View

Similar questions and discussions
How can I get a procedure for extraction pleurotus oyster polysaccharide by water extraction?

How can I get a procedure for extraction pleurotus oyster polysaccharide by water extraction?

25 November 2023 6,901 2 View

Maybe someone knows what is it (light microscopy, 100x)?

Good day to all! I discovered such strange objects on microphotographs of oyster Crassostrea gigas hemolymph. Maybe someone knows what is it? There were guesses that these objects were algae or...

16 November 2023 612 0 View

Which zone of the ocean supports the most productive fisheries and difference between Atlantic oysters and Pacific Oysters?

Which zone of the ocean supports the most productive fisheries and difference between Atlantic oysters and Pacific Oysters?

09 September 2023 239 6 View

Do crabs live in the Deep Ocean and difference between Pacific Oysters and Olympia oysters?

Do crabs live in the Deep Ocean and difference between Pacific Oysters and Olympia oysters?

09 September 2023 2,075 0 View

What ocean zone do crabs live in and what ocean zone do clams and oysters live in and zones that the pelagic region is divided into?

What ocean zone do crabs live in and what ocean zone do clams and oysters live in and zones that the pelagic region is divided into?

09 September 2023 8,544 0 View

Any tips for RNA extraction from stool matrix using RNeasy Mini Kit?

Product of interest: ZeptoMetrix Norovirus GI and GII External Run Controls. https://www.fishersci.com/shop/products/nattrol-gp-external-run-controls/21100555 I am trying to run the RNA...

07 May 2023 3,968 3 View

Whether there are some key factors for breaking through to the D larvae?

I wanted to understand the mechanisms of the oyster larvae and whether there were some key factors for breaking through to the D larvae.

26 April 2023 728 3 View

Isolated bacteria from marine sediment?

Hi, is there any pre-enrichment solution/method specify for marine bacteria? BPW is specific for enteric bacteria like Salmonella spp. I’m trying to isolate unknown bacteria from marine sediment...

02 December 2022 8,182 3 View

Measurement of the water paleo-temperature using the oxygen isotope on benthic foraminifera test, is it possible?

Can we use the Oxygen isotope on the benthic foraminifera test (e.g Orbitolinids) to determine the paleotemperature, in addition to Rudists and oysters?

23 November 2022 415 0 View

Need advice in Isolating E.coli from bivalves sample?

I isolated E.coli (oyster sample) from EC mug to EMB plates. I don’t know what happened to my other plate. The one with metallic green sheen is the positive control while the other plate is from...

06 August 2022 1,047 3 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close