How i can calculate the equivalent weight of 316L SS?

Actually 316L Stainless Steel is the alloy. So how i can calculate for its equivalent weight?

14 May 2020 4,434 5 View

Determination of wavelength in Raw effluent?

Is there a procedure to determine the wavelength of an industrial untreated raw effluent using spectrophotmeter

24 February 2020 1,931 3 View

PEG-400 or PEG-4000 can be used as an alternative to PEG-1000 ?

I use PEG-1000 (poly ethylene glycol) in my synthesis process, but I only have PEG-400 and PEG-4000. So, what is an alternative to PEG-1000 ?

19 February 2020 2,142 3 View

Why is there an unrealistically low PM2.5 concentration estimated using WRF-Chem V3.9.1?

I am conducting experimental simulations to study the PM 2.5 concentration.I have tried several chemistry options ranging from RADM2 MADE/SORGAM to MOZCART aerosol chemistry. The simulation...

21 October 2019 8,154 4 View

What are all the possible reasons for Spontaneous symmetry breaking in a double quantum well system?

Dear frnds, From my point of view SSB is happening due to following reasons in double quantum wells. 1. Due to variational technique 2. symmetry in envelop function and...

19 October 2019 6,248 2 View

Can we consider hemagglutination assay for dengue virus as a quantitative method???

For a pure virus, HA can be considered as a way of representing their titre. even though it is cost effective and less labor intensive, it is less concerned. Kindly guide me in this matter.

04 July 2019 3,881 1 View

What is the significance of size dependent dielectric screening in optimizing excitonic properties?

Mott-Wannier excitons are formed in a semiconductor material as a result of light-matter interactions. However, their delocalizing properties inside the crystal induce screening effects in their...

10 February 2019 6,737 1 View

What approach can be used to teach fraction concepts and the approach need to be based on constructivism theory?

Hi I need help for my thesis. My thesis is about teaching fraction concepts for primary pupils and I struggling to identify the appropriate constructivism learning approach.

17 June 2018 5,152 8 View

Methods for purification of DNA after digestion?

05 May 2017 178 4 View

Is there any user friendly software is available to perform interaction or docking between DNA with Isopropanol?

Is there any user friendly software is available to perform interaction or docking between DNA with Isopropanol?

27 July 2016 151 7 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View

Is there a way to extract total RNA and protein from cell culture at the same time, without denaturing protein?

Hi all, Is there a way to extract RNA and protein from the same cell culture sample? Because we need to use the protein for gelatin zymography at later time, we are looking for a method that can...

22 February 2021 5,341 5 View