I am trying to understand the different phosphorylation states of SRSF1 protein in the cytoplasm vs nucleus and looking for specific antibodies for the phosphor state. As SR proteins include various SRSF proteins I do see anti-phospho SR antibody clone 1H4 which can detect all SR phospho states. I am wondering if it only detects hypo or hyper SR proteins and it can distinguish between the two states or else different phospho antibodies are available to study this.
Thanks in advance!
you can also use the phospho tag gel...
https://doi.org/10.1016/j.jprot.2022.104558
Thank you Didier Poncet This is for total phospho site detection right? I am more interested in antibodies that are more specific to distinguish the hypo vs the hyper if they are available. Will the phospho tag gel be more specific?
no; it is an acrylamide gel that separates the different forms of your phospho proteins then you make a western blot (it is a kind of isoelectrophocusing) .
- Badges
- Science topic
- Similar topics
- Geoscience
- Asia
- India