Cell lysis protocol for Picogreen DNA assay?

I'm trying to quantify the amount of DNA in adherent cells, does anyone have a good protocol for cell lysis to prepare samples for Picogreen DNA assay? Any help will be greatly appreciated! Gloria

04 May 2016 1,438 2 View

Can you measure amount of beta-tubulin protein with respect to beta-actin?

Both beta-actin and beta-tubulin are frequently used as load controls in WB, but can I measure the amount of beta tubulin in my sample and normalized with respect to beta-actin? In other words,...

04 May 2016 2,367 2 View

What type of brain cancer cell line is most vulnerable to T-cells?

I heard most brain cancer cells avoid t-cells very well, but is there a cell line (human or animal) that is a little more vulnerable to T-cells than others? thanks! Gloria

07 August 2015 446 3 View

How do you keep PC12 cells separate from each other?

PC12 cells easily form clumps, is there a way to prevent this clumping ? Thanks, Gloria

06 July 2015 6,563 2 View

How do you get subclonal of differentiated PC12 cells?

how do you get subclonal cell line of well differentiated PC12 cells? Thanks!

06 July 2015 1,542 2 View

Is there a way to transfer live cells, without freezing them for several hours?

Other than using a portable incubator, is there a way to transfer cells without freezing for many hours?

04 May 2014 3,444 14 View

What is a solvent for folic acid?

What is a solvent for folic acid?

04 May 2014 1,893 16 View

Can you get >100% cell viability in OD readings using MTT assays and other such assays?

I would like to know if you can get more than 100% cell viability using MTT assays. Thanks!

02 March 2014 308 6 View

Can anyone help with non-surface markers for THP1 cells?

What are good non-surface markers that I can use to characterize differentiated THP1 cells?

02 March 2014 6,977 3 View

What's good as a pre-coater for better platinum adhesion on SiO2

Any material good for enhancing platinum adhering on hydrophobic surfaces?

05 June 2013 7,999 2 View

No title

02 March 2021 3,060 3 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View