21 Questions 17 Answers 0 Followers
Questions related from Gloria Bora Kim
Both beta-actin and beta-tubulin are frequently used as load controls in WB, but can I measure the amount of beta tubulin in my sample and normalized with respect to beta-actin? In other words,...
05 May 2016 2,465 2 View
I'm trying to quantify the amount of DNA in adherent cells, does anyone have a good protocol for cell lysis to prepare samples for Picogreen DNA assay? Any help will be greatly appreciated! Gloria
05 May 2016 1,541 2 View
I heard most brain cancer cells avoid t-cells very well, but is there a cell line (human or animal) that is a little more vulnerable to T-cells than others? thanks! Gloria
08 August 2015 568 3 View
how do you get subclonal cell line of well differentiated PC12 cells? Thanks!
07 July 2015 1,627 2 View
PC12 cells easily form clumps, is there a way to prevent this clumping ? Thanks, Gloria
07 July 2015 6,701 2 View
What is a solvent for folic acid?
05 May 2014 2,124 16 View
Other than using a portable incubator, is there a way to transfer cells without freezing for many hours?
05 May 2014 3,552 14 View
What are good non-surface markers that I can use to characterize differentiated THP1 cells?
03 March 2014 7,099 3 View
I would like to know if you can get more than 100% cell viability using MTT assays. Thanks!
03 March 2014 418 6 View
Any material good for enhancing platinum adhering on hydrophobic surfaces?
06 June 2013 8,091 2 View
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03 March 2013 8,510 3 View
Does anyone know how to measure sheet resistance of a material (polymer solution) using potentiostat?
03 March 2013 9,498 13 View
PEO is water soluble, but how do you dissolve PEG in organic solvent such as chloroform?
09 September 2012 6,507 22 View
Can I dissolve water-soluble PEDOT:PSS nanoparticle solution in chloroform?
09 September 2012 7,560 6 View
How should I prepare my samples in this case?
07 July 2012 186 5 View
What is the size of glucose oxidase enzyme?
07 July 2012 6,114 4 View
which neuronal cell line is RELATIVELY the cheapest (in terms of cell price itself+medium+additives) and easiest to handle and culture? But still representative to study the interaction between...
07 July 2012 5,991 2 View
I'm trying to find a method of reusing plastic cuvettes for drug release (dexamethasone) study. My method is treating the water-cleaned plastic cuvettes under UV light for some time and reusing...
06 June 2012 6,060 7 View
PEG is known to be hydrogel polymers, but they degrade in water without addition of other chemicals. UV polymerized PEG hydrogel will eventually disintegrate in water, can we still call this a...
06 June 2012 3,939 7 View
I'm thinking oxygen plasma treatment, but other than that, is there a way to make platinum hydrophillic?
06 June 2012 5,481 9 View
Amperometric biosensors display changes in current corresponding to changes in the concentration of an analyte. For in vitro biosensing where analytes will be provided to a batch of PBS for...
06 June 2012 5,892 10 View
