Cell lysis protocol for Picogreen DNA assay?

I'm trying to quantify the amount of DNA in adherent cells, does anyone have a good protocol for cell lysis to prepare samples for Picogreen DNA assay? Any help will be greatly appreciated! Gloria

04 May 2016 1,438 2 View

Can you measure amount of beta-tubulin protein with respect to beta-actin?

Both beta-actin and beta-tubulin are frequently used as load controls in WB, but can I measure the amount of beta tubulin in my sample and normalized with respect to beta-actin? In other words,...

04 May 2016 2,367 2 View

What type of brain cancer cell line is most vulnerable to T-cells?

I heard most brain cancer cells avoid t-cells very well, but is there a cell line (human or animal) that is a little more vulnerable to T-cells than others? thanks! Gloria

07 August 2015 446 3 View

How do you get subclonal of differentiated PC12 cells?

how do you get subclonal cell line of well differentiated PC12 cells? Thanks!

06 July 2015 1,542 2 View

Is there a way to transfer live cells, without freezing them for several hours?

Other than using a portable incubator, is there a way to transfer cells without freezing for many hours?

04 May 2014 3,444 14 View

What is a solvent for folic acid?

What is a solvent for folic acid?

04 May 2014 1,893 16 View

Can you get >100% cell viability in OD readings using MTT assays and other such assays?

I would like to know if you can get more than 100% cell viability using MTT assays. Thanks!

02 March 2014 308 6 View

Can anyone help with non-surface markers for THP1 cells?

What are good non-surface markers that I can use to characterize differentiated THP1 cells?

02 March 2014 6,977 3 View

What's good as a pre-coater for better platinum adhesion on SiO2

Any material good for enhancing platinum adhering on hydrophobic surfaces?

05 June 2013 7,999 2 View

Sheet resistance measurement with multimeter?

Does anyone know how to measure sheet resistance of a material (polymer solution) using potentiostat?

02 March 2013 9,299 13 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View