How do you keep PC12 cells separate from each other?

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Nai-Kei Wong

You can consider supplementing your culture with 0.5 to 1 mM sodium pyruvate.

It reduces cell clustering and uneven growth, based on lab observations.

Raj Anttal

https://www.researchgate.net/post/Having_difficulty_in_counting_PC12_cells_by_hemocytometer_as_clumps_were_still_there_I_have_tried_vigorous_pipetting_Can_anyone_help

This link will help you to solve your problem.