Hello everyone,
We aim to study the effect of certain extract on immunity. In other hand, we want to know whether our extract can boost immunity in human?
So, in first step, we should choose the suitable human cell line and then study immune activity and also immune-related gene expression.
I would be grateful if anyone could guide me or help me with other suggestions for the study.
Hello Reyhaneh Ravanbakhsh
There are human and mouse cell lines available usually derived from lymphomas or leukemias, representing all types of innate and adaptive immune cells (T and B lymphocytes, monocytes, macrophages, dendritic cells, eosinophils, basophils, neutrophils, NK, etc.).
You could use THP-1 cell line, a human leukemia monocytic cell line. This cell line could be used as a model to estimate modulation of monocyte and macrophage activities. THP-1 has unique characteristics as a model to investigate/estimate immune-modulating effects of compounds in both activated and resting conditions of the cells.
Another cell line you could use is the mouse cell line RAW 264.7, a peritoneal leukemic cell line with macrophage morphology. RAW264.7 shares some functions with primary macrophages, for instance phagocytosis, but substantially differs from primary cells in other aspects, such as spontaneous proliferation (which is high in RAW264.7 and absent in primary cells), tumoricidal activity and IL-1β production (significant in primary macrophages and absent in RAW264.7 cells). Thus, RAW 264.7 cell line could be a good in vitro model for macrophage phagocytosis.
Although the response of cell lines could hint at potential responses that could occur ex vivo or in vivo, you should always validate the results by in vivo studies to draw more definite conclusions.
You may want to refer to the articles attached below for more information.
https://pubmed.ncbi.nlm.nih.gov/25130606/#:~:text=Evaluation%20of%20recent%20papers%20leads,resting%20conditions%20of%20the%20cells.
Article In Vitro and In Vivo Models to Assess the Immune-Related Eff...
Best.
Dear Malcolm Nobre,
Thank you very much for your time and valuable answer.
Are there other suitable cell lines for this study? I found a cell line named NK-92. Do you think it is common to study this type of cell line, especially for my goal?
The best,
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