Question about lncRNA-miRNA interaction

Hi everyone, I have a question about How we can predict miRNA binding sites on lncRNAs. Some papers recommend generally some websites such as miRanda, TargetScan or PicTar, but I think none of...

14 July 2017 5,757 3 View

How can I cultivate MDA-MB-231 cell line?

Hello everybody,I am going to cultivate MDA-MB-231 cell line along with three other cell lines. I want to know if I can cultivate this cell line with RPMI-1640 Medium under the following...

21 June 2016 628 7 View

How can I cultivate MDA-MB-231 cell line?

Hello everybody,I am going to cultivate MDA-MB-231 cell line along with three other cell lines. I want to know if I can cultivate this cell line with RPMI-1640 Medium under the following...

21 June 2016 4,129 17 View

Can anyone please share a protocol for extracting exosomal RNA from serum?

Hi everyone, I am trying to extract exosomal RNA from serum using ExoQuick kit but I'm not getting any RNA from it, can anyone please tel me how to extract exosomal RNA from serum and is this kit...

06 January 2015 3,343 17 View

How can I isolate WBC for RNA extraction?

Can anyone please share a protocol for isolation of white blood cells and extraction of RNA from these cells (RNA isolation from these cells only)?

16 April 2014 9,467 11 View

Can I get the product of 320bp for real time PCR with SYBR green?

Can anybody please guide me whether I can use SYBR green for the product of 320bp or not? I heard that the best product size is usually less than 200bp ( 80-170 bp), but I think a 320bp product...

14 April 2014 8,757 13 View

How can I isolate a given protein as an intact protein by flow cytometry?

I would like to isolate a given protein as an intact and functional protein by flow cytometry (without protein unfolding or denaturation), can I do that?

06 April 2014 9,691 3 View

Can anyone please share a protocol for the extraction of total RNA from sticky cell lines using the RNAXplus Kit?

I cannot properly isolate sticky cell lines for the extraction of total RNA . I am getting low concentrations of RNA after extraction (poor bands on the agarose gel).

18 March 2014 9,374 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View