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Hello everyone, We intend to detect WSD (white spot disease) in infected shrimps (post larva and broodstock). We need to use accurate, very sensitive, rapid detection and of course cost effective...
18 November 2022 1,993 9 View
Hello everyone, I am recently doing research on Artemia Urmiana and going to study genetic diversity using ISSR. I think for designing related primers I need to know about the type of...
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Hi everyone, I have a question about How we can predict miRNA binding sites on lncRNAs. Some papers recommend generally some websites such as miRanda, TargetScan or PicTar, but I think none of...
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Hello everybody,I am going to cultivate MDA-MB-231 cell line along with three other cell lines. I want to know if I can cultivate this cell line with RPMI-1640 Medium under the following...
22 June 2016 4,187 17 View
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Hi everyone, I am trying to extract exosomal RNA from serum using ExoQuick kit but I'm not getting any RNA from it, can anyone please tel me how to extract exosomal RNA from serum and is this kit...
07 January 2015 3,391 17 View
Can anyone please share a protocol for isolation of white blood cells and extraction of RNA from these cells (RNA isolation from these cells only)?
17 April 2014 9,520 11 View
Can anybody please guide me whether I can use SYBR green for the product of 320bp or not? I heard that the best product size is usually less than 200bp ( 80-170 bp), but I think a 320bp product...
15 April 2014 8,803 13 View
I would like to isolate a given protein as an intact and functional protein by flow cytometry (without protein unfolding or denaturation), can I do that?
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I cannot properly isolate sticky cell lines for the extraction of total RNA . I am getting low concentrations of RNA after extraction (poor bands on the agarose gel).
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