16 Questions 62 Answers 0 Followers
Questions related from Reyhaneh Ravanbakhsh
I have been working with MCF-7 recently. But I think these cells are different from the MCF-7 cells that I worked with a few years ago. Furthermore, even I don't know what these round cell on the...
14 November 2024 3,861 2 View
Hi everyone, I am going to study the effect of methanolic extraction of Artemia nauplii on cancer cells. But I could not find a suitable protocol for extracting and also how to add the extract on...
18 May 2024 8,760 1 View
Hello everyone, We aim to study the effect of certain extract on immunity. In other hand, we want to know whether our extract can boost immunity in human? So, in first step, we should choose the...
19 November 2023 5,738 2 View
Hello, We have a gene that is modulated by the binding some transcription facors (TFs). We recently found that one of these TFs is mutated and we want to know if this TF can still bind to that...
17 October 2023 6,790 3 View
Hello everyone, We intend to detect WSD (white spot disease) in infected shrimps (post larva and broodstock). We need to use accurate, very sensitive, rapid detection and of course cost effective...
18 November 2022 2,092 9 View
Hello everyone, I am recently doing research on Artemia Urmiana and going to study genetic diversity using ISSR. I think for designing related primers I need to know about the type of...
11 July 2020 1,213 0 View
Hi everyone, I have a question about How we can predict miRNA binding sites on lncRNAs. Some papers recommend generally some websites such as miRanda, TargetScan or PicTar, but I think none of...
15 July 2017 6,056 3 View
Hello everybody,I am going to cultivate MDA-MB-231 cell line along with three other cell lines. I want to know if I can cultivate this cell line with RPMI-1640 Medium under the following...
22 June 2016 4,301 17 View
22 June 2016 773 7 View
Hi everyone, I am trying to extract exosomal RNA from serum using ExoQuick kit but I'm not getting any RNA from it, can anyone please tel me how to extract exosomal RNA from serum and is this kit...
07 January 2015 3,477 17 View
I would like to study Alzheimer but I don't know which cell lines is suitable for it. Can anyone please guide me? Thanks for answers in advance.
27 December 2014 6,648 16 View
Can anybody please explain to me why mitochondrial DNA is particularly susceptible to mutation? I know that in the most organisms mtDNA lacks intron and repetitive DNA. Can anybody explain...
18 April 2014 3,976 6 View
Can anyone please share a protocol for isolation of white blood cells and extraction of RNA from these cells (RNA isolation from these cells only)?
17 April 2014 9,617 11 View
Can anybody please guide me whether I can use SYBR green for the product of 320bp or not? I heard that the best product size is usually less than 200bp ( 80-170 bp), but I think a 320bp product...
15 April 2014 8,939 13 View
I would like to isolate a given protein as an intact and functional protein by flow cytometry (without protein unfolding or denaturation), can I do that?
07 April 2014 9,842 3 View
I cannot properly isolate sticky cell lines for the extraction of total RNA . I am getting low concentrations of RNA after extraction (poor bands on the agarose gel).
19 March 2014 9,521 3 View
