C2C12, 3T3-L1, L-6 and HepG2: among these, which cell line will be ideal for in vitro glucose uptake activity?
Depends on your scientific question. If your study is focused on adipose tissue then use 3T3L1, if your study regards muscle tissue then you can use L-6.
Good luck
I have used skeletel muscle cells (L6) and 3T3-L1 cells for glucose uptake, using 2-NBDG. I found that 3T3-L1 cells are more sensitive than L6 cells. So, I recommend that you use 3T3-L1 cells.
Thanks Dr. Eirini, my study regards to muscle tissue only.
As Dr. Eirini mentioned, my study is focused on muscle tissue. so i think the use of L-6 only ideal.
You can also use C2C12 however, it depends a bit on the stimuli (they respond to insulin and DNP in my hands). I reccoment using the GLUT4 myc tagged L6 cell line if you go for the L6.
L6 and 3T3-L1 cells. are the best among them.however C2C12 you can use as many studies have been reported. But former two are easy for glucose uptake. I used HEK-293 and H9C2 cells even for the glucose uptake activity with 2-NBDG:
Development of a cell-based nonradioactive glucose uptake assay system for SGLT1 and SGLT2.
Abhinav Kanwal, Shailendra Pratap Singh, Paramjit Grover, Sanjay Kumar Banerjee
PMID 22796500
Abstract
Sodium-dependent glucose cotransporters (SGLT1 and SGLT2), which have a key role in the absorption of glucose in the kidney and/or gastrointestinal tract, have been proposed as a novel therapeutic strategy for diabetes and cardiomyopathy. Here we developed a simple cell-based, nonradioactive method for functional screening of SGLT1 and SGLT2 inhibitors. Stable cell lines expressing human SGLT1 and SGLT2 were established by transfecting HEK293 cells with vectors (pCMV6-Neo) having full-length human SGLT1 and SGLT2 and selecting the positive clones following neomycin treatment. We confirmed the gene expression of SGLT1 and SGLT2 by reverse transcription polymerase chain reaction (RT-PCR) and immunoblotting. Furthermore, to analyze the function of SGLTs, we incubated stable cell lines with 2-deoxyglucose or fluorescent d-glucose analog (2-NBDG) and performed glucose uptake assay. A significant (P
Hi!. I should use a cell line whith the glucose transporter GLUT2, which is insulin-independent. The two only cell lineages having insulin-independent glucose uptake are hepatocytes and pancreatic beta cells. Apart from all the cells, that have GLUT1 which is involved in basal activity. Then, i should use or primary cultures from hepatocytes or pancreatic beta cells (primary culture or insulinoma cell lines, such as INS1E, MIN6, etc...) Good luck!!
Be careful about which L6 you are using Amyra Clip from Toronto L6 have more Glut4 than the regular L6. I advise these ones.
hi guys I found here lots of expert to find my answer i am really interested in vitro glucose uptake assay(a quick check that if my transfected gene expression in vitro changes localization of glucose receptors(glut) but in neural cells.any idea? where i should start or is there any already commercial assays out there to help me? i read about CHO cell line already available as commercial assay or sth but i am not sure i can use it to find my answers.Thanks for your help
thanks for your answer in fact no but a question came to me right now that i should first express glut-gfp receptor in this cell line for more easier microscopy technique then my special treatment(if that is the correct reason) or do simply a hybridization by GLUT Ab to see what is happening to endogenous Glc receptor?tnx
I think all the cell lines mentioned for glucose uptake assay depending on the basis of your experiment. C2C12 is an intestinal cells, HepG2 is liver cells whereas L6 is a rat skeletal muscles with different glucose transporter, pathways and mechanisms. It actually depends on your interest.
Kidney cell lines does not involve in glucose uptake as regards diabetes assay.
I would like to suggest you to go for L6 cell lines as you mentioned earlier that you wanna study on glucose metabolism. But 3t3-L1 cells are more sensitive to insulin and they show higher glucose uptake than L6 cell Lines. Please do decide your targeted tissue and choose either of the two cell lines depending on your objectiveof the study. Hope this helps
@Sunday Oyedemi Just a correction, but C2C12 are not intestinal cells. They are mouse skeletal muscle cells.
Some articles reported the assay in HEK293 cells but the process seems to happen through GLUT1 and not GLUT4. Worth trying!
What cell lines are used for Sulfonylureas ? as there primary mode of action is at pancreatic cells !
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