Dipsikha Biswas

4 Questions 17 Answers 0 Followers

Questions related from Dipsikha Biswas

Can someone share a reliable protocol for in vivo measurement of glycogen synthesis in the skeletal muscle and heart?

I am looking for a non-NMR based (preferably) method of analyzing glycogen synthesis in the skeletal muscle and heart. Can anyone share a protocol where one has used radiolabeled glucose...

18 May 2021 7,937 2 View

How can one explain a selective increase in Glut1 mRNA levels while Glut4 remain unchanged?

This is in an in vitro model of skeletal muscle cells where insulin-mediated AKT phosphorylation is increased. Any explanation will be appreciated.

02 August 2020 9,351 0 View

How to avoid dmso dissolved inhibitor from precipitating out when added in culture media?

I have been using BT2 (benzothiophene carboxylate) and even though literature suggests that it is highly soluble in dmso, it precipitates out once added in culture media at higher concentrations....

23 May 2017 9,260 5 View

What is the difference between activation and differentiation of Thp1 cells?

I wanted to know whether PMA treatment of Thp1 cells activates them or differentiates them. Certain literature suggest that PMA treatment for 24hours activate the cells following which it can be...

01 January 1970 6,428 11 View