Abhinav Kanwal

4 Questions 7 Answers 0 Followers

Questions related from Abhinav Kanwal

Why are specific proteins or genes only expressed in the specific body part?

My question is as so many proteins/genes are present in a cell or a tissue how and why specific ones are only expressed?

21 May 2014 1,581 1 View

Protocol - How to tag a particular amino-acid in a protein?

If I want to see in my protein which amino acid is acetylated or deacetylated, then what is the procedure to Flag it? Example: Suppose one particular amino acid, like lysine present at 42, 125,...

08 August 2013 1,973 0 View

RIPA Buffer in protein lysis?

Generally I use RIPA buffer for protein lysis for western Blot from tissues and cells. But I have noticed it interferes while doing estimation of proteins by Bradford's method. However I also...

06 August 2013 5,459 3 View

What are the various techniques to see how much your protein gets phosphorylated?

I want to see the how much my protein gets phosphorylated. But its phospho-antibody is not available in the market. Is there any method except doing western blot with phospho antibody of same target?

05 August 2013 6,486 2 View