Which cancer cell line overexpresses insulin receptors?

More Rajesh Kotcherlakota's questions See All

How to heat polymer for about 130° without oil bath ?

is there any other way to heat a polymer evenly other than oil bath if so what are those and how to use it

06 August 2024 947 1 View

How to dispose off lipids waste?

I am looking for a lipid waste disposal method, keeping in mind the environmental, health and safety aspect of lipid waste. Could someone please provide guidelines or the lowest acceptable...

25 July 2024 637 5 View

When Silica gel added in acetone, Silica gel not dissolved in acetone, but acetone form a light blue color solution , why ?

17 May 2024 4,351 0 View

We extracted PAHs from soil with acetone and then we used silica gel for clean up process , but after cleanup we found blue solution. Why ?

We extracted PAHs from soil with acetone and then we used silica gel for clean up process , but after cleanup we found blue solution. if we used hexane, no color changed. Is this color compound...

16 May 2024 3,288 1 View

Is there any research group or lab that works on Swarm Intelligence?

Being a researcher in the field of swarm intelligence for more than seven years, I still haven't found any research group/ institution or research lab dedicated to swarm intelligence research....

26 April 2024 4,415 2 View

I have published a paper in a Scopus Indexed Journal recently. When i will be getting DOI ? Is DOI is essential ?

I have recently published a paper in a Scopus-indexed journal. Is DOI essential? How long will it take to get DOI ? Thanks in advance

08 April 2024 4,092 3 View

How do we solve Phase change materials with metal foam using local thermal equilibrium model and local thermal non-equilibrium model in ANSYS fluent?

composite PCM cooling

13 February 2024 2,002 3 View

How to draw equivalent structure of substrate in ADS software?

how to draw equivalent structure of substrate in ADS software?

04 February 2024 7,367 2 View

What is resistive sheet and resistive inks ?how to use/get/design resistive sheet/ink in CST software?

WHAT IS RESISTIVE SHEETS AND RESISTIVE INKS? HOW TO USE/GET/DESIGN RESISITIVE SHEETS/INKS IN CST SOFTWARE?

04 February 2024 9,192 1 View

What is cryogenic tank ?

How to use SHM in Cryogenic tank

15 December 2023 3,431 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

What is saturation solubility of insulin in saliva?? I need it to know sink condition ?

05 August 2024 4,407 0 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Abdul Khaleque

Pancreatic cancer cell line.

Breast cancer cell line

Rajesh Kotcherlakota

thank you Abdul Khaleque.. i will be great full if you can tell the cell line names..we are working with those cell lines but i need specific cell line model which highly abundant in insulin receptors on its surface...

Pernille Lund Hansen

Check MCF7. At least, insulin supplentation is neccesary for cell growth in vitro.