I have to do a sandwich coating with:
1) biotinylated BSA
2) streptavidine
3) biotinylated antibody
Which are the common conditions for the incubation regarding each step?
Buffer type and pH, time and temperature?
Thanks in advance,
Veronica
1-2 hours at room temperature or at 37C is enough for most types of Abs.
Bests
Thanks Hussein
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