Which are the incubation conditions for a biotinylated antibody with a streptavidin coating?

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Interactions between oral microbiota and oral sensory systems: impact on oral comfort and pain perception?

This research question explores the complex interactions between oral microbes and the oral sensory system. The diversity and function of oral microbial communities play an important role in oral...

09 July 2024 3,601 0 View

How to implement Photovoltaic Thermal hybrid system in MatLab code?

I am trying to implement energy balance equations for a Photovoltaic thermal hybrid system using Jacobian Runge Kutta 4. However, I am getting NAN errors in the output. Are these equations...

17 February 2024 8,314 0 View

How to implement Photovoltaic Thermal hybrid system in MatLab?

I am trying to implement energy balance equations for a Photovoltaic thermal hybrid system using Jacobian Runge Kutta 4. However, I am getting NAN errors in the output. Are these equations correct?

17 February 2024 6,025 1 View

Protein crystallography. Phaser solution with Z' = 2?

I am a small molecule crystallographer currently working with a protein crystal using CCP4, so a bit outside my comfort zone. I am looking at a protein + ligand structure. The protein without...

01 January 2024 2,104 0 View

How is it possible to get different melting curve results from two different qPCR machine but the exact same protocol and kit?

Hi everyone, i was running a real-time PCR in two different machine but using the same protocol and kit. How is it possible? Can anyone explain this to me

09 October 2023 5,400 2 View

Are compounded social determinants of health in shared and substandard housing?

I would like to know when families live in shared housing or unhealthy low income housing conditions, do these factors add additional social determinants of health?

16 August 2023 3,244 3 View

Can someone help identify the yeast or fungus stained by LCPB?

I am working on a project identifying molds grown on hard cheese. One of my plates (PDA) had growth that looked like yeast. I have been staining the different mold samples using LCPB. I attemped...

16 July 2023 637 1 View

Amadubi Rural Tourism Project in Jharkhand: Development of Project Risk Management ,What cost is the cost plan?

Amadubi Rural Tourism Project in Jharkhand: Development of Project Risk Management cost plan?

06 June 2023 8,639 1 View

Amadubi Rural Tourism Project in Jharkhand: Development of Project Risk Management project scope?

Amadubi Rural Tourism Project in Jharkhand: Development of Project Risk Management (

06 June 2023 7,093 1 View

How to calculate the CI in SynergyFinder?

Dear all, I performed binary and ternary mixtures and I'd like to calculate the CI. In each mixtures, I didn't mantaine one drug at a defined concentration, but I used scalar dilutions for each...

11 April 2023 755 0 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

How to assess the osmotic power of a substance?

Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...

06 August 2024 977 1 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

How to check pH of a high strength hydrogel?

We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...

30 July 2024 942 2 View