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Hello, I need to extract an antigen from human serum. I tried with streptavidine-coated magnetic beads and a pair of biotinylated antibodies. Then I followed the elution procedure, as recommended...
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I have to do a sandwich coating with: 1) biotinylated BSA 2) streptavidine 3) biotinylated antibody Which are the common conditions for the incubation regarding each step? Buffer type and pH,...
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Did you have any problem during the filtration of the solutions? In my experience I always have a very slow filtration during the washing of the Fmoc-deprotection solution (almost 1 hour to...
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