Veronica Baldoneschi

5 Questions 7 Answers 0 Followers

Questions related from Veronica Baldoneschi

How to avoid impurities in immunoprecipitation with magnetic beads?

Hello, I need to extract an antigen from human serum. I tried with streptavidine-coated magnetic beads and a pair of biotinylated antibodies. Then I followed the elution procedure, as recommended...

10 September 2019 7,137 4 View

How to extract pure antigen from human serum?

Hello, I need to extract an antigen from human serum. I tried with streptavidine-coated magnetic beads and a pair of biotinylated antibodies. Then I followed the elution procedure, as recommended...

10 September 2019 5,538 3 View

Which are the incubation conditions for a biotinylated antibody with a streptavidin coating?

I have to do a sandwich coating with: 1) biotinylated BSA 2) streptavidine 3) biotinylated antibody Which are the common conditions for the incubation regarding each step? Buffer type and pH,...

09 November 2018 929 2 View

Did anyone work with SASRIN resin in solid phase peptide synthesis?

Did you have any problem during the filtration of the solutions? In my experience I always have a very slow filtration during the washing of the Fmoc-deprotection solution (almost 1 hour to...

04 February 2015 9,282 6 View

Which is the stability of serine with OH protected with tBu, in basic conditions?

I used LiOH 1M (3 eq.) to hydrolyze serine methyl ester, but also -OtBu was hydrolyzed.

04 February 2015 6,528 4 View