Is there a site e.g.? Or do I have to browse the literature?
For example using Ripa buffer? Or will the immunoglobulins fall apart?
10 November 2015 4,253 1 View
I tried staining cytospins of the cell lines for immunoglobulins but frequently there is quite a lot of background. Could I use western blotting?
07 August 2015 2,961 4 View
Farage is described as Epstein-Barr virus-positive, however I cannot detect expression of EBER by in situ hybridization. Can anyone explain this? http://www.atcc.org/products/all/CRL-2630.aspx
10 November 2014 7,436 5 View
I want to induce the lytic cycle of EBV in Akata and Jijoye cell lines using TPA (tetradecanoyl phorbol acetate), sodium butyrate and IgG. I was wondering how long it takes before the induction is...
10 November 2014 6,930 8 View
Detection of EBV-encoded RNA (EBER) via in situ hybridization is the gold standard to determine whether cells contain EBV. I was wondering however whether in EBER-negative cells EBV episomes can...
09 October 2014 9,746 1 View
I do not find any (recent) publications in which such commercially available antibodies are used.
08 September 2014 7,532 4 View
Hi everyone.I've been culturing OCILY3 and OCILY10 cells in RPMI1640 with 20% FBS, 10% FBS or in advance DMEM with 10% FBS (in all cases penicillin/streptomycin and glutamax were added) but in...
06 July 2014 5,404 7 View
Normal B-lymphocytes continuously circulate through the body and although a B-cell lymphoma is not really considered as a solid tumor, it does form a mass. Reading about the epithelial-mesenchymal...
06 July 2014 7,267 8 View
I was wondering if anyone working with lymphoma could tell me which reference/housekeeping genes they include in their PCR experiments?
04 May 2014 452 4 View
Does this methanol concentration kill the EBV particles? Or what would be a better fixative?
07 August 2013 944 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...
03 August 2024 5,828 4 View
