Julie Morscio

14 Questions 14 Answers 0 Followers

Questions related from Julie Morscio

Is it possible to extract intact immunoglobulins from fresh frozen immunoglobuline-producing tumor biopsies?

For example using Ripa buffer? Or will the immunoglobulins fall apart?

11 November 2015 4,241 1 View

Can I use western blot to assess immunoglobulin expression in cell lines?

I tried staining cytospins of the cell lines for immunoglobulins but frequently there is quite a lot of background. Could I use western blotting?

08 August 2015 2,933 4 View

I cannot detect Epstein-Barr virus-encoded RNA (EBER) in the Farage cell line?

Farage is described as Epstein-Barr virus-positive, however I cannot detect expression of EBER by in situ hybridization. Can anyone explain this? http://www.atcc.org/products/all/CRL-2630.aspx

11 November 2014 7,409 5 View

How long does the effect of lytic cycle-inducing agents (TPA, sodium butyrate, IgG) last on Epstein-Barr virus (EBV)-producing cell lines?

I want to induce the lytic cycle of EBV in Akata and Jijoye cell lines using TPA (tetradecanoyl phorbol acetate), sodium butyrate and IgG. I was wondering how long it takes before the induction is...

11 November 2014 6,918 8 View

Can the Epstein-Barr virus (EBV) be episomally present in B-cells without associated expression of viral RNA or protein?

Detection of EBV-encoded RNA (EBER) via in situ hybridization is the gold standard to determine whether cells contain EBV. I was wondering however whether in EBER-negative cells EBV episomes can...

10 October 2014 9,717 1 View

Are there are any antibodies available - other than anti-BZLF1/ZEBRA - to detect lytic EBV by immunohistochemistry?

I do not find any (recent) publications in which such commercially available antibodies are used.

09 September 2014 7,509 4 View

Does anyone have experience with culturing OCILY3 and OCILY10 cells?

Hi everyone.I've been culturing OCILY3 and OCILY10 cells in RPMI1640 with 20% FBS, 10% FBS or in advance DMEM with 10% FBS (in all cases penicillin/streptomycin and glutamax were added) but in...

07 July 2014 5,367 7 View

What is known about metastasis of B-cell lymphoma?

Normal B-lymphocytes continuously circulate through the body and although a B-cell lymphoma is not really considered as a solid tumor, it does form a mass. Reading about the epithelial-mesenchymal...

07 July 2014 7,242 8 View

What is the best reference/housekeeping gene for PCR on lymphomas?

I was wondering if anyone working with lymphoma could tell me which reference/housekeeping genes they include in their PCR experiments?

05 May 2014 437 4 View

Where can I find information regarding chemosensitivity/resistance of lymphoma cell lines?

Is there a site e.g.? Or do I have to browse the literature?

08 August 2013 6,518 0 View

Is 30-60% methanol (like Thinprep PreservCyt) a safe fixative for EBV-infected cells?

Does this methanol concentration kill the EBV particles? Or what would be a better fixative?

08 August 2013 917 0 View

Does anyone have a protocol to stain fixed lymphoma cells?

I would like to fix my cultured cells and perform immunohistochemistry.

02 February 2013 4,176 0 View

Where can I buy plasmids encoding Epstein-Barr Viral proteins (LMP1, EBNA2, etc.)?

For (non-viral) transfection.

01 January 2013 3,095 1 View

Can anyone tell me if there are commercially available lymphoma cell lines of which there is an Epstein-Barr Virus-positive and -negative variant?

I focus on aggressive lymphoma (Diffuse large B-cell Lymphoma, Burkitt Lymphoma). By the variants I mean eg. an Epstein-Barr-Virus(EBV)-negative Burkitt cell line that has been in vitro...

01 January 2013 5,973 13 View