In the literature it says that you can keep DNA samples preserved in DMSO/EDTA/NaCl mix for several months at room temperature? That's like 20-22C. What if ambient temperature is like 26-30C when doing field work in the tropics for example?
Cheers
As a general rule isolated DNA can be stored at 4oC for several weeks, at -20oC for several months and at -80oC for several years.
Goodluck
But i am using DESS (DMSO/EDTA/NaCl). literature says you can store at room temp for several months using this technique
I once left DNA samples (PCR products) purified and dissolved in water at room temperature (that is 28-30*C over here) for a month, and found no apparent degradation when I checked them -not that I'm recommending doing so, of course. But my point is, if it -apparently- worked in water, it probably works in DESS.
You can keep it at 25- 30 degrees Celicius for up to three months. There will be a slight reduction of the volume due to evaporation don't wait until it is all evaporated; you may need to dilute it with the DNA elution buffer prior to use, however you have to be very careful with what you use it for as your DNA will have a higher salt concentration.
Thank you guys! I will desalt the sample in TE buffer before extraction.
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