11 November 2012 2 6K Report

Recently, I met a tricky system.

The proteins made from TNT.T7 reticulocyte lysate are sensitive to freezing and thawing steps.

I was assuming the denatured protein might be more stable than regular stuff.

But, even though I tested different ways to store them, the 2nd run for SDS-PAGE is completely different to 1st run.

I have a protease inhibitor inside. I tried to snap freeze my samples. I re-boiled my samples before loading.

Has anyone experienced something similar?

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