5 Questions 26 Answers 0 Followers
Questions related from Quan Li
Recently, I met a tricky system. The proteins made from TNT.T7 reticulocyte lysate are sensitive to freezing and thawing steps. I was assuming the denatured protein might be more stable than...
11 November 2012 6,300 2 View
I am measuring miRNA levels with qPCR using taqman probe (regular, no detailed information on it). Recently I introduced some miRNA with GFP-tag to my system, and did qPCR with the taqman...
10 October 2012 6,556 11 View
I have protein (GFP) tagged miRNA, and want to isolate the complex from whole cell lysate. My target is miRNA, not protein. Since there's only small amount of miRNA expressed in my system, I was...
10 October 2012 7,002 15 View
I am using windows 7, and trying to install cygwin1.dll and cygz.dll. I got lots of error alarm during software installation.
10 October 2012 6,836 1 View
Does anybody know how to install and use context likelihood of relatedness (CLR) algorithm to identify potential mRNA targets as mentioned in the article below? I want to do the same thing, but...
09 September 2012 6,991 2 View
