I’m planning to investigate in nano control area via plant tissue culture. I read about various methods. I'd like some advice about recommended techniques.
It will depend on the virus you are studying. For instance PVY (and many other potato viruses) can be mechanically transmitted, so you could infect the plants simply by wounding the plantlet and exposing the wound to infected material. PLRV on the other hand needs infectious aphids to transmit the virus.