Please advise if there is any opportunity to participate in the project as a co-author. thank you, Elham

More Elham Metwally's questions See All

Promotor observation in region annotation of RNA RIP-seq?

Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...

18 July 2024 1,579 2 View

Why do I get no melt curve peak and no Ct for some of my patients but not others, in a SYBR green qPCR?

I am doing a RT-qPCR for gene expression analysis of cancer patients, and there are four different groups in the results. The first group that shows over expression of my target gene (both two...

31 May 2024 1,088 0 View

Can anyone interpret why the absorbance in alpha amylase assay get increased with increased standard (inhibitor) concentration?

Can anyone interpret why the absorbance (DNS assay method) in alpha amylase assay get increased with increased standard (inhibitor) concentration? it should be decreased with increased standard or...

26 May 2024 4,753 2 View

Anyone with phosphate flotation experience?

I attempted a phosphate (apatite) flotation, but the slurry was too thick for the impeller to work properly. I reduced the solid content to 30% for better mixing, but the conditioning still seems...

14 May 2024 4,505 4 View

Why my amino acids do not contribute in amidation reaction?

Hello everyone, recently I am trying to synthesize a dipeptide with a Coumarin derivative of Tryptophan, and the problem is it doesn't contribute in reaction at all. Tryptophan itself in the same...

15 April 2024 6,778 3 View

How to increase concentration and quality of RNA after extraction?

Hello All, I'm doing RNA extraction from cells. I tried both ways by using TRIZOL (with chloroform, and isopropanol) and RNeasy mini kit. When I use TRIZOL, the RNA concentration is high, however...

11 March 2024 9,171 8 View

How to interpret my samples' results from D1000 ScreenTape Assay?

Hello All I prepared my library to run for sequencing. I would like to check the quality of my samples through D1000 ScreenTape Assay before add to sequencer. Can anyone help me to interpret my...

10 March 2024 8,764 0 View

Can anyone help with SHSY5Y cells differntiation?

Hello all I'm working with SHSY5Y cells to differentiate them to neuronal cells. I'm following the protocol with using RA, BDNF and cAMP for cell differentiation. I tried it different times but...

19 February 2024 1,672 5 View

Why Cryo cells (freezed cells) do not attached to the flask?

Hello all I'm working with SHSY5Y cells and I always made Cryo cells from them, them thawed them and used them without problem. But, recently, after thawing, they are remain floating and not...

19 February 2024 9,305 2 View

Has anyone experienced a significant A after 15 min. of thiol disulfide exchange reaction and then no A could be detected?

In thiol-disulfide exchange reaction, pyridine-2-thione is expected to be released as the reaction proceeds giving yellow color to the medium, and the Absorbance should increase and then become...

15 January 2024 689 0 View

Why do exism movements become permanent dictatorship threats within liberal democracy thinking under majority rule-independent rule of law system?

Exism movements after gaining power within liberal democracies under majority rule and independent rule of law system become permanent dictatorship threats, but why this is the case is not clear...

04 August 2024 8,125 3 View

Would you like to join our meta analysis research team?

I am excited to announce an opportunity for dedicated researchers to join our dynamic team for an ongoing meta-analysis study. If you are passionate about research and have a keen interest in...

01 August 2024 6,737 1 View

What is effective targeted chaos?

Perfect democracy thinking assumes no chaos so no need for independent rule of law system and liberal democracies assume the possibility of normal democratic chaos that can be sorted out by an...

28 July 2024 473 1 View

Why is effective targeted chaos more than emocracy?

Exism movements since BREXIT 2016 have been described as driven by emotions leading to the idea of Emocracy/Emocracies, but as the social discontent that is usually displayed after exism movements...

16 July 2024 3,701 1 View

¿Should a pilot study be done before conducting a systematic review?

14 July 2024 3,036 3 View

can I use Roc Curve in a pilot study?

Hi everyone, we know that the analysis of pilot studies should be based on descriptive statistics and ideally not involve any inferential statistics (i.e., P values). Based on this can I use Roc...

24 June 2024 4,402 7 View

Difference b/n Pilot study and exploratory study?

The Analyse for Pilot study should be based on the descriptive stat and ideally not involved inferential stat. what about exploratory study? can I do inferential stat like cox regression or...

17 June 2024 5,470 3 View

Minimum sample size for a mix method pilot study?

What is the minimum sample size for a pilot study in a mix method research?

28 May 2024 1,044 8 View

How to find the original questionnaire of the article-Dimensional analysis of schoolchildren’s food label comprehension: a pilot study?

27 May 2024 2,551 0 View

Pilot study inclusion in a retrospective chart review?

What is the best practise with regards to pilot study data being included in the final data analysis when conducting a restrospective chart review.

25 May 2024 4,498 2 View