You should consider using ion chromatography. Column should be 270x7.8mm, particle size 10micro meters. Polystyrene/divinylbenzene copolymer packing. The eluent should be a mixture of NaHCO3 and Na2CO3. Good luck.
ORGANIC ACIDS PROFILE IN TOMATO JUICE BY HPLC WITH UV DETECTION
OMBRETTA MARCONI, SIMONA FLORIDI, LUIGI MONTANARI
can u give details of conditions like which mobile phase, what is the flow rate and what is he range of wavelength and the column used @simona floridi
You might want to try HILIC chromatography, works very well with polar compounds. Compatible with UV, MS and other detectors. Typically, ACN, water, and a buffer, eg. ammoium acetate 10 mM.
See e.g. this site:
http://www.merckmillipore.com/INTL/en/analytics-and-sample-preparation/education-support-and-events/literature-chromatography/zic-hilic-hplc-guides/VySb.qB.UgkAAAFEfikfWwxs,nav
The ideal conditions of HPLC for organic acids detection depend on several factors, namely the kind of sample to be analysed, the solvent(s) used for the extraction of compounds, and the target compounds. Please see the following article:
- Canas et al (2014). Phytochemical Analysis. DOI 10.1002/pca.2526
For simple short organic acids (lactic, acetic, citric, etc.) in aqueous samples... I use a Bio-Rad Aminex HPX-87H column running a very dilute sulfuric acid mobile phase (ultra pure water + acid) with a UV detector set at 200-210 nm. Supelco also makes a similar "H" column. These work well. Remember to buy the matching guard columns to protect the main column. These columns work with almost any standard HPLC machine and UV detector (flow rates about 1ml/min, degas the solvent). You can also us an refractive index detector if you don't have uv/vis.
Also, these columns work for simple sugars and short chain alcohols too. If that is in your sample, you can detect those with an refractive index detector.
For long chain fatty acids, you will need to use a different column and setup, post back if you need to do longer chain fatty-acid analysis (ie oleic acid).
Use of ion-exclusion chromatography for monitoring fatty acids produced by bacterial anaerobic degradation of tetrachloroethene in ground water.
N Chamkasem
Narong Chamkasem 11.90 · 21.05
U.S. Food and Drug Administration
Journal of Chromatography A (Impact Factor: 4.61). 02/1991; 536(1-2):193-201. DOI: 10.1016/S0021-9673(01)89249-9Source: PubMed
Chandandeep: I am happy to see you are interacting with other scientists though Research Gate. Carry on with good work.
Dear Nicholas, I am using similar method in my analysis for organic acids as well...do you mind sharing some papers regarding to the method you mentioned above ? It could be really helpful for me...tq
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