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Questions related from Steven Ray Wilson
Does anybody have some good papers on peptide peak area between-sample repeatability after treatment with trypsin (i.e. bottom-up proteomics)? Needs to be for complex samples, and not just...
04 July 2017 4,625 2 View
In Norway, there is a debate regarding the use of asthma medicine in sports. The debate was fueled by the doping violation of the cross-country skier Martin Sundby. It has also been stoked by the...
10 September 2016 3,039 3 View
Hi everybody! I could really use a list of endogenous human metabolites with traits (most interested in hydrophobicity, charge and Mw). Does anybody know if this exits? I want to use it to attempt...
16 September 2015 7,549 3 View
Hi friends, looks like I might need to look at some metabolites in exosomes floating around in blood. I need good suggestions for what to do/not do for isolating the exosomes. Some of the blood...
22 April 2015 3,361 4 View
Coming from a background where internal standards is an absolute must for quantification, I wonder about spectral counting in proteomics. How can this work properly, considering ion suppression...
19 March 2015 5,235 6 View
I need to do some proton NMR of approx. 20-30 samples. Do you know of any good and simple ways to prepare the samples for 1H NMR (cell washing, counting, lysing, NMR solutions)? I can have approx....
14 February 2015 8,513 3 View
Hi everyone! We have found that a mass spectrometry based technology we are working on can do really fast measurements of targeted proteins. We would like to demonstrate the technology with...
31 January 2015 8,815 9 View
Hi Everyone! How much of a between-sample variance in retention time would you accept in a targeted proteomics assay (when analysing similar samples)?
10 December 2014 2,011 7 View
There is rather scarce information in scientific literature on practical, day to day handling of tracheomalacia in children, e.g. the effect of humidifiers, temperature, and physical activity....
17 November 2014 8,561 2 View
LC is/has been traditionally associated with mm-scale inner diameter columns, packed with 3-5 um particles. But today, we have access to e.g. UHPLC columns, monolithic columns, in all shapes and...
17 October 2014 5,448 12 View
Nano LC (0.05-0.1 mm ID columns) is the "weapon of choice" in mass spectrometry based proteomics research. It can be an absolute necessity when analyzing very small amounts of sample. Although...
12 October 2014 6,660 22 View
Identification of an unknown substance (e.g. a plant metabolite or an MS peak that your PCA analysis implies has predicitive value) can be tricky stuff. What steps would you say are...
08 October 2014 2,044 24 View
Two papers recently published in Nature describe the human proteome (1, 2). But the protein identification procedures in these have recently been seriously criticized (3). I believe this is an...
16 July 2014 8,794 3 View
