What would be the best protocol for MTT assay to check cytotoxicity in U87MG cells?

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What basic protocol can you recommend for making liposomes with a highly water insoluble drug?

I am new to liposome making. My drug (carbamazepine, mol wt. 236) is highly insoluble in the water. I tried to load it in bilayer using a basic protocol. After lipids(DMPC, DMPG, Cholesterol)...

23 November 2020 4,886 3 View

Does anyone has experience with Dextran-FITC uptake by cancer cells?

Please share the protocol for antigen uptake assay by cancer cells. I want to use Dextran-FITC to track the endocytosis by cancer cells. If anyone has the protocol or any suggestions kindly share.

25 October 2020 469 3 View

Can crude enzyme be used for immobiliazation?

Can crude enzyme be utilized as immobilized enzyme for industrial application if the crude enzyme shows high activity?

13 July 2020 8,672 4 View

From where can I get microglia cell line like N9 or BV-2 in India ( preferably in Kolkata)?

05 November 2019 7,719 2 View

The right statistical test for microbiological data from food production environment?

I am analyzing the safety aspects of two food production sites producing similar products. I wish to compare these geographically distinct sites across seasons as well as processing steps. I have...

15 July 2019 550 3 View

Can anyone please suggest me code of LEACH algorithm in python.?

02 July 2019 5,023 1 View

How to conjugate a dye to the N-terminus of poly-glutamic acid peptide?

Hi! I am trying to conjugate a dye in its NHS ester form to the N-terminus of poly-glutamic acid peptide ( which is already available in the form of a white solid). I tried 3 times and am tring...

27 June 2019 8,798 3 View

Nuclear fractionation protocol for ChIP-sequencing and Co-IP mass spectrometry?

The protein I am working on is a transcription factor and is abundant in nucleus. I want to do subcellular fractionation of the adherent tumor cells followed by ChIP sequencing in order to...

02 December 2018 5,978 1 View

Is it necessary to purify PCR products before cycle sequencing?

I will purify the cycle sequencing products using EDTA/EtOH precipitation method before loading to 96 well plate.

30 November 2018 6,932 4 View

Please suggest me the method to determine the thickness of coating on whole fruits and vegetables?

How can be the thickness of coating on fresh whole fruits and vegetables be determined?

04 November 2018 4,296 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

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28 February 2021 9,936 2 View