Sakshi Goswami

9 Questions 1 Answers 0 Followers

Questions related from Sakshi Goswami

What kind of contamination is this?

I am working with U87MG cell line and I got contamination in one of the flasks. Can anyone tell me what type of contamination is this?

22 September 2023 8,654 0 View

Measurement of Reactive oxygen species (ROS) in Blood using DCFDA dye ?

I want to measure reactive oxygen species in whole blood. Can anyone please suggest a protocol or method for it using 2',7'-dichlorodihydrofluorescein diacetate (DCFDA) dye ?

10 February 2023 3,581 1 View

How to get MCF 7 cells to adhere in the culture flask ?

I am working with MCF 7 cell line. I ordered the cell line from NCCS Pune. I received a very high confluent flask. After a day, I splitted the cells. I am using MEM w/Earle's salts, 2mM...

16 December 2021 4,148 4 View

What is the protocol for protein extraction and protein estimation in cell culture ?

Can someone please provide me with the detailed protocol for protein extraction from cells using RIPA lysis buffer and the estimation of protein by bradford assay. I need to perform western blot...

26 August 2021 2,404 4 View

What kind of contamination is this ?

I am working with U87MG (glioblastoma) cell line. I am using MEM with NEAA+ 1mM Na Pyr+ FBS as media. Today I found some sort of contamination in the cells. Some of the cells were detached from...

02 August 2021 5,839 2 View

Why the cells keep detaching from the side wells of a well plate ?

I am working with U87MG (glioblastoma) cell line. I am using MEM with NEAA+ 1mM Na Pyr+ FBS as media. The cells grow fine in T-25 flasks. But when I seed them in a 96 or 12 well plate, the cells...

29 July 2021 8,917 2 View

Why my U87MG (glioblastoma) cells keeps losing their morphology?

I am currently working with U87MG (glioblastoma) cells. I am using MEM with NEAA+ 1mM Na Pyr+ FBS as media. The cells grow fine in T-25 flasks. But sometimes, when I check them, they seem to lose...

27 July 2021 5,674 3 View

What would be the best protocol for MTT assay to check cytotoxicity in U87MG cells?

I am currently working with U87MG cell line (glioblastoma). I need to perform MTT assay to check cytotoxicity of a compound. I have came across so many protocols of MTT assay. Which would be the...

15 July 2021 7,789 3 View

I am working with U87mg cell line. They grow fine in T-25 flasks using MEM but why they keep getting detached from the surface of 12 well plate ?

I am currently working with U87mg cell line. I am using MEM with NEAA+ 1mM Na Pyr+ FBS as media. The cells grow fine in T-25 flasks. But whenever i seed them in 96 or 12 well plate, they gets...

01 June 2021 6,159 1 View