Dear All,
I am trying to analyse targeted metabolites i.e Indole-3-acetic acid, IPA and ILA from fecal samples by HPLC. I have came across several extraction protocols for GC-MS but nothing on HPLC. Any help would be greatly appreciated.
I propose to make an aqueous solution from the feces and then, centrifuge, clean and enrich using SPE C18 columns, then subject to HPLC analysis, preferably with a fluorescence detector. In a similar way, we determine indole derivatives in brassica plant samples according to the procedure from the publication: Pilipczuk, T., Dawidowska, N., Kusznierewicz, B. et al. Simultaneous Determination of Indolic Compounds in Plant Extracts by Solid-Phase Extraction and High-Performance Liquid Chromatography with UV and Fluorescence Detection. Food Anal. Methods 8, 2169–2177 (2015). https://doi.org/10.1007/s12161-015-0106-x
- Badges
- Science method