How does bis(4-nitrophenyl)-phosphate (BNPP) act in chemotherapy?

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What would be the best method to isolate RNA from Caco2 cell monolayer grown on 24 wells transwell inserts?

Hi, I am growing Caco2 cells in 24 Transwell cell inserts. I am planning to induce gut leakage by LPS and want to study gene expressions. My concern is to isolate the RNA from transwell inserts...

01 April 2024 5,759 3 View

Why my acceleration and displacement is not changing with speed when I am performing moving load analysis in SAP2000 ultimate?

I want to see the variation of speed on acceleration. For that I have considered a simply supported beam subjected to constant moving force. I am performing time history -direct integration...

16 January 2024 166 0 View

How to perform Energy Decompostion Analysis of an open shell system?

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08 October 2023 9,223 0 View

Why mouse intestinal organoid started dying suddenly?

Mouse intestinal organoids were growing fine until 3 passages. After that, I passages them last Friday and left for the weekend. Monday I found that they were dying. I am not sure what is the...

03 September 2023 501 2 View

Please Share Protocol for transformation ~9KB CRISPR Plasmid Using dh5alpha cells?

I have been preparing competent dh5alpha cells in the lab with good competency not excellent. however, have not been able to transform my CRISPR plasmid yet. I am following all the desired steps...

16 July 2023 1,898 3 View

What would be an optimized protocol to analyze indole derivatives from fecal sample by HPLC?

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29 January 2023 9,720 1 View

How do I add different concentrations of Riboflavin to my media (DMEM) for cell culture use?

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22 January 2023 7,405 2 View

What should be the dilution factor to test LPS concertation in Serum and Fecal Sample by ELISA?

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04 January 2023 2,425 1 View

What is the best solution to identify and reverse the negative sequences in FASTA files for PICRUST2 ?

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13 November 2022 8,025 1 View

Why I am not able to get MS2 plaques?

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12 December 2021 2,528 3 View

Is changing the medium during the experiment overestimate the results?

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07 August 2024 2,283 2 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

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Radiogenomics Cancer Research Challenges?

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03 August 2024 5,828 4 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

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03 August 2024 2,730 1 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

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How to maintain the drug concentration in cell culture study for long term?

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24 July 2024 8,696 0 View

For a case-control study for Breast Cancer incidents in a particular County in USA, what data can be used as Control? E.g. colorectal cancer?

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22 July 2024 9,954 4 View

About Machine Learning for Automated Diagnosis of Skin Cancer with Dermoscopy Images ?

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How can I determine if a drug binds to a ribosome using a Cryo-EM map?

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Fixation Problem with cancer cells?

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Cristiano Ferlini

BNPP is a specific inhibitor of carboxylesterase (CE). BNPP affects the metanolism of chemo such as Topo-I inhibitors and has been used in animal models to modulate the effects of chemotherapy when CE activity is high.

http://imr.sagepub.com/content/33/1/84.full.pdf

Nupur Raychaudhuri

Thank you Cristiano.