Hello..

It is not uncommon for the optimal dilution factor for an ELISA assay to vary depending on the sample type and the specific assay being used. Therefore, it is generally recommended to perform a dilution series to determine the optimal dilution factor for your specific samples and assay.

To determine the optimal dilution factor for mouse serum and fecal samples, you can start by preparing a series of dilutions of your samples using a suitable diluent, such as phosphate-buffered saline (PBS). For example, you could prepare serial dilutions of your samples ranging from undiluted to 1:10, 1:100, and 1:1000. Then, you can perform the ELISA assay using each of these dilutions and compare the results to determine the optimal dilution factor.

As for extraction of LPS from fecal samples, there are several methods that have been described in the literature. One commonly used method is the phenol-water extraction method, which involves adding a phenol-water mixture to the sample, followed by centrifugation to separate the LPS from other components of the sample. Other methods that have been used to extract LPS from fecal samples include the EDTA extraction method and the hexadecyltrimethylammonium bromide (CTAB) extraction method.

It's worth noting that the efficiency and effectiveness of these methods can vary depending on the specific characteristics of the sample and the LPS being extracted. If you are having difficulty extracting LPS from your fecal samples, I recommend consulting a scientist or expert in the field to discuss potential solutions.

regards .