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Questions related from Samnhita Raychaudhuri
I have been growing Caco2 cells well for one year. I have never faced any issue with that. The media composition is MEM+20%FBS+1% antibiotic. Recently, I have been experiencing problems with...
18 October 2024 7,359 3 View
Hi, I am growing Caco2 cells in 24 Transwell cell inserts. I am planning to induce gut leakage by LPS and want to study gene expressions. My concern is to isolate the RNA from transwell inserts...
02 April 2024 5,791 3 View
Mouse intestinal organoids were growing fine until 3 passages. After that, I passages them last Friday and left for the weekend. Monday I found that they were dying. I am not sure what is the...
04 September 2023 520 2 View
Dear All, I am trying to analyse targeted metabolites i.e Indole-3-acetic acid, IPA and ILA from fecal samples by HPLC. I have came across several extraction protocols for GC-MS but nothing on...
30 January 2023 9,748 1 View
Dear All, I am testing on the concentration of Lipopolysaccharide in mouse serum and fecal samples by ELISA. I didn't find much information on the dilution factor for both types of samples for...
05 January 2023 2,444 1 View
Hello all, I am having an issue with 16S PICRUST data. There is always a warning message post PICRUST run that more than half of the sequences have been removed from further analysis. The reason...
14 November 2022 8,044 1 View
Hello all, I have run one SDS PAGE gel with 40ug of protein / well from mice adipose tissue. But I see some incomplete beta actin bands on my membrane. Please advice me. Transfer method : Wet...
07 August 2020 9,142 9 View
