Samnhita Raychaudhuri

6 Questions 4 Answers 0 Followers

Questions related from Samnhita Raychaudhuri

What would be the best method to isolate RNA from Caco2 cell monolayer grown on 24 wells transwell inserts?

Hi, I am growing Caco2 cells in 24 Transwell cell inserts. I am planning to induce gut leakage by LPS and want to study gene expressions. My concern is to isolate the RNA from transwell inserts...

02 April 2024 5,786 3 View

Why mouse intestinal organoid started dying suddenly?

Mouse intestinal organoids were growing fine until 3 passages. After that, I passages them last Friday and left for the weekend. Monday I found that they were dying. I am not sure what is the...

04 September 2023 518 2 View

What would be an optimized protocol to analyze indole derivatives from fecal sample by HPLC?

Dear All, I am trying to analyse targeted metabolites i.e Indole-3-acetic acid, IPA and ILA from fecal samples by HPLC. I have came across several extraction protocols for GC-MS but nothing on...

30 January 2023 9,744 1 View

What should be the dilution factor to test LPS concertation in Serum and Fecal Sample by ELISA?

Dear All, I am testing on the concentration of Lipopolysaccharide in mouse serum and fecal samples by ELISA. I didn't find much information on the dilution factor for both types of samples for...

05 January 2023 2,442 1 View

What is the best solution to identify and reverse the negative sequences in FASTA files for PICRUST2 ?

Hello all, I am having an issue with 16S PICRUST data. There is always a warning message post PICRUST run that more than half of the sequences have been removed from further analysis. The reason...

14 November 2022 8,042 1 View

Why I am getting incomplete bands in western blot ?

Hello all, I have run one SDS PAGE gel with 40ug of protein / well from mice adipose tissue. But I see some incomplete beta actin bands on my membrane. Please advice me. Transfer method : Wet...

07 August 2020 9,139 9 View