09 October 2023 0 9K Report

Want of to perform EDA of an open shell system, Pu(NO3)4.2H2O, here Pu has 4 unpaired electron. Please provide a detailed answer with a procedure of preforming the same in ADF.

More Diganta Raychaudhuri's questions See All
What would be the best method to isolate RNA from Caco2 cell monolayer grown on 24 wells transwell inserts?

Hi, I am growing Caco2 cells in 24 Transwell cell inserts. I am planning to induce gut leakage by LPS and want to study gene expressions. My concern is to isolate the RNA from transwell inserts...

01 April 2024 5,759 3 View

Why mouse intestinal organoid started dying suddenly?

Mouse intestinal organoids were growing fine until 3 passages. After that, I passages them last Friday and left for the weekend. Monday I found that they were dying. I am not sure what is the...

03 September 2023 501 2 View

What would be an optimized protocol to analyze indole derivatives from fecal sample by HPLC?

Dear All, I am trying to analyse targeted metabolites i.e Indole-3-acetic acid, IPA and ILA from fecal samples by HPLC. I have came across several extraction protocols for GC-MS but nothing on...

29 January 2023 9,720 1 View

What should be the dilution factor to test LPS concertation in Serum and Fecal Sample by ELISA?

Dear All, I am testing on the concentration of Lipopolysaccharide in mouse serum and fecal samples by ELISA. I didn't find much information on the dilution factor for both types of samples for...

04 January 2023 2,425 1 View

What is the best solution to identify and reverse the negative sequences in FASTA files for PICRUST2 ?

Hello all, I am having an issue with 16S PICRUST data. There is always a warning message post PICRUST run that more than half of the sequences have been removed from further analysis. The reason...

13 November 2022 8,025 1 View

Why I am getting incomplete bands in western blot ?

Hello all, I have run one SDS PAGE gel with 40ug of protein / well from mice adipose tissue. But I see some incomplete beta actin bands on my membrane. Please advice me. Transfer method : Wet...

06 August 2020 9,122 9 View

How can we reduce surface water runoff in refugee camp that seem to affect the camp occupants a lot ?

Thank you all for your thoughts and responses to my previous question on what are the most serious  problems in refugee camp. The question and the responces can be found in the following...

13 April 2016 5,123 4 View

What are the most serious issues/problems in a large refugee camp?

You may have heard of or seen a number of refugee camps. In recent times, I have heard of the refugee camps a lot in various contexts. In your opinion what are the most serious issues / problems...

06 April 2016 8,132 8 View

What is role of Chemical and Environmental Engineering in the Transport Sector?

There has been significant development in Transport Sector in recent time, e.g., development of autonomous vehicles. What is role of Chemical and Environmental Engineering in these sector?

18 March 2015 8,822 6 View

Hi all, does Erlotinib affect the binding of adaptin to membrane receptor proteins?

Erlotinib is a potent inhibitor of EGFR activated signal transduction

19 November 2014 8,646 0 View

Similar questions and discussions
How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

1. If I can quantize the atom using this hyperbolic spiral and classical physics, could nature do the same?

If we map as a continuous motion an ionising electron (beginning its journey at n=1) in an H atom, a specific hyperbolic spiral appears (see animation). When we solve this spiral formula, we find...

07 August 2024 5,343 2 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

Which distribution type should I use when calculating the average particle size from TEM image? and how to calculate the error ?

average particle size calculation from TEM

04 August 2024 2,921 1 View

Why reactivity isn't increased with more empty spots in valence shell?

If from a geometric perspective the non-halogens, non-noble gases have more empty spots in their valence shell, and the filling/exiting of any of the empty spots in the shell constitutes a...

03 August 2024 4,787 2 View

Why is the molecule's orientation with an electric field affect polarizability?

Why is the molecule's orientation with an electric field affect polarizability? Electrons are diffuse enough to be independent with respect to orientation and effect of electric field on...

03 August 2024 7,843 1 View

Why doesn't chromium 2+ ion use all its d-orbitals to receive lone pairs from six waters in [Cr(H20)6)]3+?

I'm guessing it's because the ligand experiences too much electron repulsion or proton repulsion from the chromium to insert them close to the 3d-orbitals which are close to the metal nucleus. Is...

03 August 2024 1,370 1 View

Is it necessary to covary exogenous constructs in a structural model?

I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...

03 August 2024 6,028 4 View

Why aren't most metal oxides insoluble in water?

Why BaO (s) + H2O(l) ---> Ba 2+(aq) + 2 OH - (aq) if Oxygen loves owning all the electrons, why would it bind to hydrogen which competes with Oxygen for electron ownership more so than...

03 August 2024 5,392 1 View

Why do paired electrons atoms produce no magnetic field lines?

If you think of electrons with spin as bar magnets, you know bar magnets of opposite polarity as long as they're not occupying the same spatial location don't cancel out each other's magnetic...

03 August 2024 3,927 2 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close