Hi all, does Erlotinib affect the binding of adaptin to membrane receptor proteins?

20 November 2014 0 9K Report

Erlotinib is a potent inhibitor of EGFR activated signal transduction

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What would be the best method to isolate RNA from Caco2 cell monolayer grown on 24 wells transwell inserts?

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Why my acceleration and displacement is not changing with speed when I am performing moving load analysis in SAP2000 ultimate?

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16 January 2024 166 0 View

How to perform Energy Decompostion Analysis of an open shell system?

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08 October 2023 9,223 0 View

Why mouse intestinal organoid started dying suddenly?

Mouse intestinal organoids were growing fine until 3 passages. After that, I passages them last Friday and left for the weekend. Monday I found that they were dying. I am not sure what is the...

03 September 2023 501 2 View

Please Share Protocol for transformation ~9KB CRISPR Plasmid Using dh5alpha cells?

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What would be an optimized protocol to analyze indole derivatives from fecal sample by HPLC?

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How do I add different concentrations of Riboflavin to my media (DMEM) for cell culture use?

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22 January 2023 7,405 2 View

What should be the dilution factor to test LPS concertation in Serum and Fecal Sample by ELISA?

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04 January 2023 2,424 1 View

What is the best solution to identify and reverse the negative sequences in FASTA files for PICRUST2 ?

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13 November 2022 8,025 1 View

Why I am not able to get MS2 plaques?

Hello, I had purchased MS2 (freeze-dried) from ATCC: 15597-B1 (https://www.atcc.org/products/15597-b1). I revived the culture according to the method given in the product sheet. For propagation, I...

12 December 2021 2,528 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to fix errors in my heat transfer steel structure with reinforced concrete slab model Abaqus?

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How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

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Why activated CAR-Jurkat cell could not kill targets?

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06 August 2024 640 2 View

How to isolate lymphocytes from mouse spleen?

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04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

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04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

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04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

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03 August 2024 3,851 1 View