i am going to make a native page gel for total protein in plants. so, do i need a protein marker
Check this discussion, I think it answers your question:
https://www.researchgate.net/post/Is_using_a_protein_ladder_necessary_for_native_gel_electrophoresis_If_yes_is_there_a_special_ladder_for_native_PAGE2
Molecular weight markers for native gels are commercially available:
https://www.thermofisher.com/order/catalog/product/LC0725
https://tools.thermofisher.com/content/sfs/manuals/NativeMarkUnstainedProteinStnd_man.pdf
Generally speaking, however, protein molecular weights can't be assessed accurately by a single native PAGE because charge and shape also affect the migration. There is an old method for doing this using multiple native gels that vary in acrylamide percentage.
Dear Anandita,
To summarize, a single native gel is inconclusive since you have to account for charge and size. A molecular weight marker does not account for charge. If you have already purified proteins of your sample, that can provide you with some insights on the presence of those specific proteins.
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