The slow growth of H9C2 cells and the formation of cell clumps after centrifugation and resuspension might be caused by what factors?
I’m trying to reproduce the acousto-optic coupling simulation in the paper Frequency–angular resolving LiDAR using chip-scale acousto-optic beam steering. LNOI(lithium niobate on insulator) is...
13 August 2024 9,795 0 View
Request Python code from this article : Gender equity of authorship in pulmonary medicine over the past decade. THANKS!
08 August 2024 6,242 2 View
One of my friends, every night after falling asleep, his mouth would make a creaking sound after chewing something. This teeth grinding condition would continue until he woke up, and he didn't...
08 August 2024 1,366 5 View
It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...
28 July 2024 7,983 2 View
Hi there, I'm new to modelling with comsol. I would like to ask if it is possible for comsol to output linearly polarized (LP) modes? I tried modelling a simple single mode fiber with an enlarged...
23 July 2024 4,019 3 View
Hello! We are using LNP to deliver circular mRNA into T cells. However, compared with linear mRNA, the transfection efficiency and protein expression duration are similar. Are LNP generation or...
21 July 2024 4,322 1 View
when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.
17 July 2024 3,532 0 View
What is the future scope of acoustic emission?
17 July 2024 1,510 1 View
I read two papers which uses Instantaneous SINR (ISINR) as criterion for performance evaluation. In the two papers below, it seems the desired signal (numerator) in ISINR does not involve...
16 July 2024 4,545 0 View
I want to extract basement shear force in LS-DYNA calculation result, but i found that there were no force results but only moment results in SPCFORC even I check the model and make sure the...
23 June 2024 2,691 0 View
I am designing research related to atherosclerosis in vitro, but unfortunately the cell lines currently available in our laboratory are only 3T3-L1 and H9C2, Does anyone can suggest me what...
17 July 2024 6,373 1 View
Hello everyone, We needed cardiac cells for a project and obtained H9C2 rat cardiovascular myoblast cells from another laboratory. The cells are at passage 15 and unfortunately have...
07 October 2023 7,589 1 View
Can anyone suggest me a pre-sorting and post-sorting buffer for H9C2 cardiomyocytes cell lines? I'm using BD Facs Melody Thanx
29 November 2022 6,899 0 View
Hi all, I tried many times to measure the glucose uptake using florescent 2-NBDG, but unfortunately, all failed. After seeding the cells, I incubate the cells (overnight incubation) with low...
21 November 2021 3,421 2 View
I'm working with H9C2 cells for the first time and I've been following protocols I could find on papers and research gate. For the sub-culture, I seed 10k cells/cm2 and split the cells every 2-3...
22 August 2021 1,884 3 View
I try to transfect H9C2 cells that are differentiated into cardiomyocytes by daily RA stimulation. However, when I try to transfect them using Lipofectamine, it appears that only undifferentiated...
17 March 2021 861 1 View
Does anyone have a detailed and reliable protocol for culturing, seeding and preparing H9c2 cells for whole-cell patch-clamp? What's the best practice to prepare H9c2 for single cell...
25 January 2021 2,444 0 View
I have been trying to thaw my frozen H9C2 cells but none of the cells attach to the flask, I had some other cell lines stored along with my H9C2 vials, they are thawing very well. I tried changing...
04 July 2020 4,832 0 View
Can anyone kindly help me with the best way to induce hypertrophy in H9C2 cardiomyocyte cell? If yes, kindly suggest the ideal protocol for it. Thanks and looking forward for your kind response.
09 October 2019 196 2 View
I am starting working with H9C2 cells and I am not sure how they were cultured before so I would like to reclone them to have a sure population of myoblasts.
03 February 2019 4,477 0 View