We assessed the ability of 50 age-matched insect lines/populations from 30 diverse geographic regions to survive infection. Median lethal times (LT50) were monitored using five replicates (20-30 insects each) per sex per insect line, and experiments were repeated at least twice.

I did correlation analyses between insect LT50 and maximum temperatures, which were collected from 30 global locations. At 30-35C maximum temperature, there are 0 or 1 days at 1/3 of localities. Is it acceptable if the values of some independent variables are 0 or 1 day?

Some locations have more than one insect line collected, especially 10 insect lines from Tokyo, Japan. We want to know whether there are correlations/differences between LT50 and maximum temperature at 30 geographic regions/localities. There are ten pairs of X, Y variables for the one Tokyo, Japan location. Should we use the average LT50 of 10 lines from Tokyo or without pooling?

Another question, we tried to divide 50 insect lines into 8 biome groups, each group has 3-13 insect lines. Should we compare the group LT50 by using the original 10-15 replicates of each of 50 insect lines with replicate N numbers, or the mean LT50 of insect lines with line N numbers in 8 groups?