As the culturing protocol says, to grow the 3T3-L1 cells in DMEM in presence of calf serum (Newly born calf serum-NCS) and for the differentiation experiments, people suggest to use Fetal calf serum (FCS). What will happen if i use Fetal Bovine Serum (FBS) for culturing 3T3-L1 preadipocytes?
"Bovine calf serum (BCS, Calf Serum) is obtained from healthy, inspected calves 2 weeks to 12 months of age. Although Bovine Calf Serum has a lower nutritional value than Fetal Bovine Serum or Newborn Bovine Calf Serum, it can be used for a variety of less demanding cell lines."
Hi,
I have used FBS for pre-adipocyte (3T3-L1) differentiation and the cells were too good. Good luck
We used the same FBS for 3T3-L1 preadipocytes and adopocytes. We were able to differentiate the adipocytes up to 80-90% without any problems. More critical in our hands was the fresh preparation of the chemicals required for differentiation.
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