Johan Renes

2 Questions 14 Answers 0 Followers

Questions related from Johan Renes

Does anybody have experience with TGF-beta 1 - induced migration of HT-29 cells?

We have grown HT-29 cells (serum free) on non-coated transwells (8 um pore size, Greiner Bio) and incubated these at the basolateral side with 2.5, 5 and 10 ng/ml TGF-beta 1 for 12-24-48-72 hrs....

31 March 2015 992 11 View

Does anyone have experience with isolating separate total lipid and protein fractions from adipose tissue biopsies?

I am eager to receive some tips or even protocols on best practices.

11 June 2014 7,350 3 View