How do check multi toxin concentration ?

More Karthikeyan Periyasamy's questions See All

How to filter few markers from a gene set?

I am not good in bioinformatics. I have a gene set which has some 1000 genes which are highly correlating with aging. I want to select some highly significant genes from this geneset for further...

04 November 2020 5,602 5 View

Base paper for renewable energy forecast especially wind energy?

Please suggest me a good base paper for forecasting renewable energy. I am interested in doing research on applying deep learning neural network model for forecasting. Thank you

17 November 2019 2,234 5 View

Where can I get wind energy data for carrying out forecasting research?

Dear Researchers, I am started exploring forecasting research work for renewable energy forecast especially wind. I want to develop a DNN model for forecasting of wind energy. So I need a standard...

14 November 2019 3,854 7 View

What will be the perfect markers to differentiate Neural Stem cells from differentiated Neurons?

I've been working on differentiating Rat fetal Neural Stem cells into Neurons with a drug of interest. The Problem here for me is I have used various Neuronal markers like MAP2, TUJ1, NFM to...

15 October 2019 7,114 5 View

How to extract elevation value related to a particular pixel (latitude, longitude) in USGS 1-arc-second SRTM Geotiff file using python3?

Attached the elevation data from USGS 1-arc-second SRTM data. I understood that every pixel is associated with a latitude/longitude and elevation data at that location. I want to extract the...

30 August 2019 7,094 3 View

What is the difference between fuzzy optimization and mechine learning optimization? In which kind of application, we can prefer fuzzy optimization?

Difference between fuzzy optimization and mechine learning optimization.

13 August 2019 9,248 1 View

While creating analytical fields using expressions in Abaqus, what will the unit of theta if we use trigonomtetric function?

I have applied pressure as trigonometric function using the expression cos(2*atan(Y/X)). I would like to know what will the unit of atan(Y/X). Is it in Degree or Radian?

10 July 2019 8,518 3 View

Why the SOD1 mutant model expressing the mutant protein but not the native protein?

I am working with drug activity on familial ALS model cells. I am using mouse Neural stem cells which express the mutated human SOD1 protein. I did western blot with the protein extracted from the...

14 March 2019 511 3 View

How to use fatigue tool for composite in a Ansys workbench?

Currently doing final year project in a leaf spring fatigue test in Ansys workbench 16.0. we have done fatigue test for steel, but error came for composite while testing we don't know how to...

07 March 2019 3,100 2 View

Crack initiation and propagation in Fe-safe?

Whether crack initiation and propagation in the finite element model could be obtained from the results of Fe-safe? Is it possible to know how the crack propagates?

30 January 2019 7,736 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

How to interpolate from a time-series database ?

Hi, I am having trouble with a problem, in the field of Optimal Control and the generation of optimal time-series. Let's consider a system, whose dynamics are represented by dx/dt =...

24 February 2021 9,105 2 View

Hi i want to ask that in order to find out LC 50 (96 hours) of particular toxin for fish. Should we keep the animals with food or without food?

if we expose the fish after acclimatization to particular toxin in order to find out LC 50 (96 hours).Should we keep the fish off feed or with feed for that duration. Some literature show that...

24 February 2021 357 2 View

How to run raise regression in R?

if anybody know how to run raise regression in r, and raise parameter selection, please help me

24 February 2021 8,199 1 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

Hi all, My research questionnaire has 3 sections each having a mix of yes/no and multiple choice questions. How can I test its reliability ?

The sections are about awareness, practice, and perception regarding waste management. Thanks

20 February 2021 5,722 3 View

How to dilute Toxin-DMSO solution?

I have a concentrated solution of a toxin(50 micromolar) in DMSO 100%. I need to dilute it inorder to dilute both the toxin and DMSO wich can be toxic to cells. Which would you guys recommend to...

19 February 2021 7,447 2 View

How to perform Kaplan-meier survival analysis in SPSS?

Hi everyone, This is my first time of attempting to run survival analysis. I have a data on patients with End-stage Kidney Diseases (ESKD). I would want to run survival probability by treatment...

16 February 2021 2,647 3 View

What is the best way to calculate sample size for a multivariate regression model for adequate power?

GPower is only equipped to deal with calculations of bivariate regressions and needs SD s for both predictor samples. However, if one has a multiple regression with more than 2 outcome variables...

12 February 2021 6,911 3 View

Which statistical method should i use to compare the independent variables of 2 linear regressions?

I have an enormous dataset and for each row, I have a predicted value, and in the same row, there are a few characteristics(independent variables). I have set an ideal linear regression for this...

11 February 2021 8,761 6 View

Karthikeyan Periyasamy

(PDF) Purity Assessment of Crystalline Zearalenone (researchgate.net)

Jose Troestch

Dear Karthikeyan, I think that the ideal analytical technique would be a chromatographic method. Particularly with mass spectrometry, or in the absence of that possibility, fluorescence detection has been applied successfully on multiple occasions to determine the mycotoxins of interest.

As a clean-up and extraction method, the use of specific immunoaffinity columns for AFs, OTA and ZON would be ideal; although QueChers and SPE are also options.

I share some titles of papers that may be useful.

1. Validation of the procedure for the simultaneous determination of aflatoxins ochratoxin A and zearalenone in cereals using HPLC-FLD A.

Food Additives & Contaminants: Part A: Chemistry, Analysis, Control, Exposure & Risk Assessment

Rahmani, S. Jinap & F. Soleimany

Article Validation of the procedure for the simultaneous determinati...

2. Determination of Mycotoxin Contamination Levels in Rice and Dietary Exposure Assessment. Journal of Toxicology.

Jose Troestch, Stephany Reyes and Aracelly Vega

Article Determination of Mycotoxin Contamination Levels in Rice and ...

I hope this information is useful.

Jose Troestch Thanks, sir it is useful information, and we know about lit bit toxin extraction and quantified the toxin level in different raw materials and feed, we did TLC and HPLC analysis. but I need how long it's sustainable to use multi-toxin std. toxin std self-life.

Ok, sorry for my confusion. For the verification that you mention, the "molar absorption coefficient" is used. You can find this procedure in an AOAC manual and this article can also be useful.

Toxins (Basel). 2020 Feb; 12(2): 94. doi: 10.3390/toxins12020094

Stability of Mycotoxins in Individual Stock and Multi-Analyte Standard Solutions

I hope to be of help this time.