What is the acceptability limit of CV % of intermediate precision ?

in pharmaceutical feild : an anlytical method is valid according to the classical approch (descriptive), when the relative coefficient of variation of intermediate precision is less than 3%. in...

31 December 2018 2,092 0 View

Evaluating data stability Following ICH Q1(E) ?

ICH guidline ask for the justification of presentation of new drug substance, to do so we should follow the ICH Q1(E) guideline, This guideline is destinated to evaluate data stability indeed,...

02 March 2018 522 7 View

Why mean responses are used to define surface response?

in the second (optimisation) step of application of design of experiment, we tried to set a model to define most influential factors"we call too surface response methodology" , in parallel, this...

09 October 2017 8,989 2 View

How can we try the system suitability test ?

my question is about the place of system suitability test in developing and validating a New analytical UPLC Chromatography method. its places is considered as a step in the analytical validation...

09 October 2016 283 10 View

Reversed phase liquid chromatography ?

is reversed phase liquid chromatography used only in gradient elution ? what i know we use in reversed phase liquid chromatography (hydrophobic mobile phase)

09 October 2016 160 10 View

What is System suitability test ?

02 March 2016 9,151 3 View

What is Incurred Samples?

09 October 2015 4,080 1 View

The result of measurement of uncertainty of method should big a small?

in each laboratory analyst are obliged to give a state of uncertainty of their measurement, so in this way the value of uncertainty should be so correct to create a certain reliability between...

08 September 2015 4,880 1 View

How can accuracy profile integrate three parameters of validation as linearity, trueness and precision?

05 June 2015 5,687 3 View

How can we define a acceptance limits in validation studies?

02 March 2015 119 6 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Microstrip antenna design tutorial of HFSS 3D layout module ?

I have designed a 24 GHz rectangular antenna on HFSS 3D model and got desired results while desing the same antenna with same dimensions I am getting resonance frequency at 27 GHz I am...

28 February 2021 4,426 2 View