How can i find catalytic domain for specific protein kinase using cDNA ?

07 August 2016 8,217 0 View

Transfection should I grow the cells in Media without antibiotic and FBS ?

Transfection should i grow the cells in Media without antibiotic and FBS ?

05 June 2015 9,263 5 View

Please help I am new in Plasmid construction ?

04 May 2015 3,281 3 View

How can the cell attach to cell culture plate?

How can the cell attach to cell culture plate? I know there are two reasons. First, electrostatic reaction second, extracellular matrix but need more explanation.

03 April 2015 6,757 4 View

Can anyone suggest why the growth of my cell culture of TNBC MBA-MD-436 is slow?

03 April 2015 4,256 3 View

Need help I just start my first cell culture experiment .what's rounded shape cells means in cell culture during the growing in media no trypsin ?

03 April 2015 2,174 7 View

Can anyone please help me to find information about these 6 cell lines ?

kuramochi -ovcar - ovarsha- HEYA8- SKVO3- OVAMANA Please help me to learn more about these cell lines.

10 November 2014 624 2 View

Why am I getting a very dark high background in WB Kifc1 antibody?

I'm working now on western blot and trying to detect Kifc1, but it gives me a dark background and I washed for 2 hrs and used 5% BSA-TBST blocking. The same problem of high background is very dark.

10 November 2014 281 5 View

What's the importance of making standard curve for protein estimation ?

i do it but i can't understand the mechanism and how we can estimation our protein using Bradford soln ?

10 November 2014 4,244 4 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View