Ahmed Mohamed Abdelbaki

10 Questions 5 Answers 0 Followers

Questions related from Ahmed Mohamed Abdelbaki

How can i find catalytic domain for specific protein kinase using cDNA ?

I want to use bioinformatic to achieve my goal

08 August 2016 8,265 0 View

Transfection should I grow the cells in Media without antibiotic and FBS ?

Transfection should i grow the cells in Media without antibiotic and FBS ?

06 June 2015 9,352 5 View

What should i expect on my SDS gel after analyzing my immunoprecipitation experiment?

I am doing immunoprecipitation using Thermo Scientific Pierce Crosslink IP Kit for specific protein and followed by SDS Page and staining with coomassie stain to send mass spectroscopy After...

06 June 2015 2,981 0 View

Please help I am new in Plasmid construction ?

How can demarcate the following details with sequences i need to understand -origin of replication-antibiotic resistance gene-restriction enzyme sites into which the HSET ORF has been cloned in...

05 May 2015 3,323 3 View

How can the cell attach to cell culture plate?

How can the cell attach to cell culture plate? I know there are two reasons. First, electrostatic reaction second, extracellular matrix but need more explanation.

04 April 2015 6,816 4 View

Can anyone suggest why the growth of my cell culture of TNBC MBA-MD-436 is slow?

I try to grow MEME having 1% pencilin/ streptomysin and 10% FBS The growth of this cell very slow Did any one grow TNBC MBA-MD-436 ? Please suggest media and how to grow them i try to grow MEME...

04 April 2015 4,307 3 View

Need help I just start my first cell culture experiment .what's rounded shape cells means in cell culture during the growing in media no trypsin ?

I need to understand the morphology of cells during cell culture .During growing cells . i found some cells rounded and not attach to substrate and other cells attach but star or rod like

04 April 2015 2,226 7 View

Can anyone please help me to find information about these 6 cell lines ?

kuramochi -ovcar - ovarsha- HEYA8- SKVO3- OVAMANA Please help me to learn more about these cell lines.

11 November 2014 678 2 View

What's the importance of making standard curve for protein estimation ?

i do it but i can't understand the mechanism and how we can estimation our protein using Bradford soln ?

11 November 2014 4,300 4 View

Why am I getting a very dark high background in WB Kifc1 antibody?

I'm working now on western blot and trying to detect Kifc1, but it gives me a dark background and I washed for 2 hrs and used 5% BSA-TBST blocking. The same problem of high background is very dark.

11 November 2014 335 5 View