How can i find catalytic domain for specific protein kinase using cDNA ?

07 August 2016 8,217 0 View

Transfection should I grow the cells in Media without antibiotic and FBS ?

Transfection should i grow the cells in Media without antibiotic and FBS ?

05 June 2015 9,263 5 View

What should i expect on my SDS gel after analyzing my immunoprecipitation experiment?

05 June 2015 2,941 0 View

Please help I am new in Plasmid construction ?

04 May 2015 3,281 3 View

How can the cell attach to cell culture plate?

How can the cell attach to cell culture plate? I know there are two reasons. First, electrostatic reaction second, extracellular matrix but need more explanation.

03 April 2015 6,757 4 View

Can anyone suggest why the growth of my cell culture of TNBC MBA-MD-436 is slow?

03 April 2015 4,256 3 View

Need help I just start my first cell culture experiment .what's rounded shape cells means in cell culture during the growing in media no trypsin ?

03 April 2015 2,174 7 View

Can anyone please help me to find information about these 6 cell lines ?

kuramochi -ovcar - ovarsha- HEYA8- SKVO3- OVAMANA Please help me to learn more about these cell lines.

10 November 2014 624 2 View

What's the importance of making standard curve for protein estimation ?

i do it but i can't understand the mechanism and how we can estimation our protein using Bradford soln ?

10 November 2014 4,244 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View