I want to use bioinformatic to achieve my goal
Transfection should i grow the cells in Media without antibiotic and FBS ?
05 June 2015 9,398 5 View
I am doing immunoprecipitation using Thermo Scientific Pierce Crosslink IP Kit for specific protein and followed by SDS Page and staining with coomassie stain to send mass spectroscopy After...
05 June 2015 2,999 0 View
How can demarcate the following details with sequences i need to understand -origin of replication-antibiotic resistance gene-restriction enzyme sites into which the HSET ORF has been cloned in...
04 May 2015 3,346 3 View
How can the cell attach to cell culture plate? I know there are two reasons. First, electrostatic reaction second, extracellular matrix but need more explanation.
03 April 2015 6,841 4 View
I try to grow MEME having 1% pencilin/ streptomysin and 10% FBS The growth of this cell very slow Did any one grow TNBC MBA-MD-436 ? Please suggest media and how to grow them i try to grow MEME...
03 April 2015 4,326 3 View
I need to understand the morphology of cells during cell culture .During growing cells . i found some cells rounded and not attach to substrate and other cells attach but star or rod like
03 April 2015 2,243 7 View
kuramochi -ovcar - ovarsha- HEYA8- SKVO3- OVAMANA Please help me to learn more about these cell lines.
10 November 2014 699 2 View
I'm working now on western blot and trying to detect Kifc1, but it gives me a dark background and I washed for 2 hrs and used 5% BSA-TBST blocking. The same problem of high background is very dark.
10 November 2014 358 5 View
i do it but i can't understand the mechanism and how we can estimation our protein using Bradford soln ?
10 November 2014 4,324 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
What are the roles of innovation in achieving the Sustainable Development Goals (SDG)?
31 July 2024 3,533 2 View
I am looking for software to help analyze SELEX NGS data, including alignment, sequence enrichment, and other related tasks. Can anyone recommend suitable tools or software? Best wishes, Waleed
30 July 2024 1,061 5 View
PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...
28 July 2024 4,762 2 View
I wanted to know whether we can observe the synergistic/antagonistic/additive properties of combinations or mixtures of compounds through docking analysis. But during docking preparation any...
28 July 2024 7,413 6 View
Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...
28 July 2024 6,938 1 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View
