Why is my SH-SY5Y not gaining a differentiated (neuronal) phenotype after treatment with 12-O-tetradecanoyl-phorbol-13 acetate (TPA or MPA).

I am treating 1-week cultured SH-Sy5Y with 80 nM TPA for one week, replacing the media with the TPA twice a week according to (Presgraves, 2004). Instead of growing neurites, I notice that the...

06 June 2013 4,329 7 View