I want to check the activity and the efficacy of 5 different drugs in cell lines to evaluate the best one based on their potency and reduced toxic effects. In many papers, researches did take the different concentrations based on the IC50 value of the drug but it is generally found that the IC50 value varies between cell lines. Is their any standard procedure to choose the concentration range? To check its efficacy, should I follow the experimentally determined IC50 value or the highest effective value?
Looking for your reply.
Thank you
Thank you so much. If any previous literature review has suggested dose range in different cases, could I take it as a reference point?
I would test a wide range of concentrations for each drug for each cell line. The high end of the concentration range might be limited by the solubility of the compound in media. You could use half-log dilutions to cover the range with a small number of dilutions (e.g. 100, 30, 10, 3, 1, 0.3, 0.1, etc µM).
Hello,
I agree with Adam, if you are working on drugs that are not reported before or limited literature is available......and your are not getting the exact idea from where to start from ......then you better select a wide range of concentration as suggested by Adam....
Good luck....
I recommended you to search more about literature of mentioned drugs and select suitable cell line for each one. Then according to suggested method by Adam start to evaluate each one. Meanwhile, you can find standard method for determination of IC50 value for your drugs.
Wish you more success
I agree with answers above, i think you should use a wide range of concentrations of each drug as suggested by Adam , also if these drugs are used by certain patients you may need to calculate and find roughly systematic circulating of the drugs and then you can use concentrations higher and lower this systematic concentration of these drugs.
Good luck.
Hello,
Should be used series concentration from drug , and I agree with Adam,
Good luck....
Hi Mayank,
I agree with Adam and Thomson. It's better if you use a wide range of drug concentrations since you have not yet come across literature using the drugs and the cell lines of your interest. I would have considered the IC50 value and along with that 3-4 concentrations above and below the IC50. And yes you should never consider the highest effective value.
Good luck!
Most people start by doing 10 fold dilutions starting in nano molar range up to high micro molar range. Once the area of activity is defined by this you then do a more precise series surrounding the effective concentrations. If no activity from molar to micro molar then probably not worth pursuing.
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