How can I do RNAseq?

I have murine B cells. I'd like to perform RNA-seq upon treatment. How much starting RNA is required to do it?Could someone shares a protocol? thanks in advance Simone

09 October 2015 6,971 0 View

Can someone suggest a protocol for SiRNA and suspension cells?

Hi guys, I'm going to perform a KD of my protein of interest. The problem is that I'm working with suspension cells. Could someone suggest a protocol avoiding electroporetion? thanks

09 October 2014 5,104 6 View

Does interferon gamma have an effect on DAUDI cell line and Interferon?

Hi, I need your expertise. I need to know if interferon gamma has effects on DAUDI cells. Thanks a lot

08 September 2014 3,641 3 View

How is it possible to eliminate polymers?

Recently I observed polymers in my samples. Do you have some suggestions to avoid their presence?

04 May 2014 8,761 7 View

Can anyone give me suggestions on doing nuclear/citoplasm fractionation?

Unfortunately when I performed N/C fractionation I assessed protein precipitation. Usually I used a hypotonic buffer to separate C from the N. On the nuclei I applied a strong saline buffer. The...

03 April 2014 3,542 4 View

Do you know which markers of Euchromatin and Heterochromatin I could use for IF?

I'm interested in understanding if my protein will colocalize with chromatin.

02 March 2014 7,775 4 View

Any suggestions for the fractionation of suspension cells?

I'm having a lot of problems performing nucleus/cyto fractionation in DAUDI cell line. The protein recovery is really low. Do you have any suggestions?

02 March 2014 2,812 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View