Suspension cells are generally best transfected with electroporation, but if you don't have access to it, try using a generic lipofection reagent (https://altogen.com/product/altofect-transfection-reagent/) and that should give you decent results (though not stellar). siRNA transfection is generally efficient, so for knockdown experiments, if you have a selection method it should work.

This question was answered by others before (link below). Your best bet is probably going to be to use a transfection reagent. I also linked Sigma's protocol for their siRNA transfection reagent that includes transfection of suspension cells.

https://www.researchgate.net/post/Is_there_any_transfection_reagent_for_knocking_down_genes_in_suspension_culture

http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Bulletin/1/s1452bul.pdf

I used INTERFERin from polyplus for siRNA transfection of Daudi B cells

Thanks a lot, I will study both approaches

Dear Simone,

I recommend Lullaby siRNA transfection reagent or DreamFect Gold

Thiose have been sucessfully used to transfect siRNA in suspensino cells such as THP-1, Jurkat, K562 etc. You can see the resutls and publications here

http://www.ozbiosciences.com/transfection-dna/19-dreamfect-gold-transfection-reagent.html

http://www.ozbiosciences.com/transfection-sirna/24-lullaby-sirna-transfection-reagent.html

Best Regards

Suspension cells are generally best transfected with electroporation, but if you don't have access to it, try using a generic lipofection reagent (https://altogen.com/product/altofect-transfection-reagent/) and that should give you decent results (though not stellar). siRNA transfection is generally efficient, so for knockdown experiments, if you have a selection method it should work.