I'm interested in understanding if my protein will colocalize with chromatin.
H3K9 trimethylation is mark of heterochromatin, Abcam has great antibodies for IF. Meanwhile H3K4 trimethylation is a nice mark for euchromatin, abcam has nice antibodies here too. There was a paper about overlapping hetero/euchromatic signatures in line elements, but there they were using as a markers H3K4 di methy for euchromatin and DNA methylation for heterochromatin. I do not know how much this would extend. But for your purposes the two marks listed above should suffice.
Hi Kristina, thanks a lot. I tested H3K4Me3 and you are totally right. It's worked properly. To asses the co-localization with the Heterochromatine I used H3K9Me3
but unfortunately it didn't work. What about HP1 for Heterochromatine? Have you never tested it?
Thanks
Hi Simone, HP1 should work as well, as a marker for heterochromatin, although I have not used it personally, I am using the H3K9Me3 from abcam, Ab8898, at 1:500 at the moment and it is really nice, which antibody did you try and what cell types?
Hi Kristina, I used the one from Santa Cruz. It's work properly. I'm working with lymphocytes... They are really, scientifically speacking, BASTARDs. It is difficult to plate them in the sense that they have difficulties to stay attached to the coverslip. I have to treat them.
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