What program do I have to use for Daudi transfection using AMAXA?

Hi guys, could you share with me your protocol to transfect DAUDI cells by AMAXA? I have to do siRNA transfection. I have the nucleofector solution V thanks

11 December 2014 4,260 1 View

Can someone suggest a protocol for SiRNA and suspension cells?

Hi guys, I'm going to perform a KD of my protein of interest. The problem is that I'm working with suspension cells. Could someone suggest a protocol avoiding electroporetion? thanks

09 October 2014 5,104 6 View

Does interferon gamma have an effect on DAUDI cell line and Interferon?

Hi, I need your expertise. I need to know if interferon gamma has effects on DAUDI cells. Thanks a lot

08 September 2014 3,641 3 View

How is it possible to eliminate polymers?

Recently I observed polymers in my samples. Do you have some suggestions to avoid their presence?

04 May 2014 8,761 7 View

Can anyone give me suggestions on doing nuclear/citoplasm fractionation?

Unfortunately when I performed N/C fractionation I assessed protein precipitation. Usually I used a hypotonic buffer to separate C from the N. On the nuclei I applied a strong saline buffer. The...

03 April 2014 3,542 4 View

Do you know which markers of Euchromatin and Heterochromatin I could use for IF?

I'm interested in understanding if my protein will colocalize with chromatin.

02 March 2014 7,775 4 View

Any suggestions for the fractionation of suspension cells?

I'm having a lot of problems performing nucleus/cyto fractionation in DAUDI cell line. The protein recovery is really low. Do you have any suggestions?

02 March 2014 2,812 3 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View