Simone Tamburri

8 Questions 24 Answers 0 Followers

Questions related from Simone Tamburri

How can I do RNAseq?

I have murine B cells. I'd like to perform RNA-seq upon treatment. How much starting RNA is required to do it?Could someone shares a protocol? thanks in advance Simone

10 October 2015 7,006 0 View

What program do I have to use for Daudi transfection using AMAXA?

Hi guys, could you share with me your protocol to transfect DAUDI cells by AMAXA? I have to do siRNA transfection. I have the nucleofector solution V thanks

12 December 2014 4,302 1 View

Can someone suggest a protocol for SiRNA and suspension cells?

Hi guys, I'm going to perform a KD of my protein of interest. The problem is that I'm working with suspension cells. Could someone suggest a protocol avoiding electroporetion? thanks

10 October 2014 5,160 6 View

Does interferon gamma have an effect on DAUDI cell line and Interferon?

Hi, I need your expertise. I need to know if interferon gamma has effects on DAUDI cells. Thanks a lot

09 September 2014 3,683 3 View

How is it possible to eliminate polymers?

Recently I observed polymers in my samples. Do you have some suggestions to avoid their presence?

05 May 2014 8,795 7 View

Can anyone give me suggestions on doing nuclear/citoplasm fractionation?

Unfortunately when I performed N/C fractionation I assessed protein precipitation. Usually I used a hypotonic buffer to separate C from the N. On the nuclei I applied a strong saline buffer. The...

04 April 2014 3,585 4 View

Any suggestions for the fractionation of suspension cells?

I'm having a lot of problems performing nucleus/cyto fractionation in DAUDI cell line. The protein recovery is really low. Do you have any suggestions?

03 March 2014 2,860 3 View

Do you know which markers of Euchromatin and Heterochromatin I could use for IF?

I'm interested in understanding if my protein will colocalize with chromatin.

03 March 2014 7,814 4 View